针灸“肺俞”“大椎”“风门”对哮喘模型大鼠肺组织STAT6、血清IL-13表达的影响  

Effect of Acupuncture and Moxibustion of BL13,DU14 and BL12 on the Expressions of STAT6 in Lung Tissue and IL-13 in Serum of Asthmatic Rats

作　　者：徐宁 邵素菊[2] 华金双[2] 王培育[2] 张君[2] 任重[2] 胡晓京 田丽 XU Ning;SHAO Suju;HUA Jinshuang;WANG Peiyu;ZHANG Jun;REN Zhong;HU Xiaojing;TIAN Li(Henan Province Hospital of TCM, Zhengzhou 450002, China;The Third Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450008, China)

机构地区：[1]河南省中医院,河南郑州450002 [2]河南中医药大学第三附属医院,河南郑州450008

出　　处：《中医药信息》2021年第4期42-45,共4页Information on Traditional Chinese Medicine

基　　金：2018河南省科技攻关项目(182102310108)。

摘　　要：目的:观察针刺和艾灸对哮喘模型大鼠血清中IL-13及肺组织中STAT6表达的影响,探讨“肺俞”“大椎”“风门”治疗哮喘的作用机理。方法:将40只SPF级SD大鼠按照随机数字表随机分为空白组、模型组、艾灸组和针刺组,每组各10只。采用卵蛋白(OVA)溶液致敏制备哮喘模型,空白组选用生理盐水代替卵蛋白造模。艾灸组和针刺组选取大鼠的“肺俞”“大椎”“风门”分别进行艾灸、针刺治疗;空白组和模型组不做治疗,进行相同抓握刺激。末次治疗后取腹主动脉血,采用ELISA法检测血清中IL-13的表达;取肺组织,用HE染色观察各组大鼠肺组织形态学改变;RT-PCR检测肺组织中STAT6 mRNA的表达。结果:HE染色显示,空白组大鼠支气管管腔规整,黏膜无明显水肿,未见到炎性细胞的浸润;模型组大鼠肺组织中可见大量炎症细胞浸润,其中以嗜酸性粒细胞、淋巴细胞为主,支气管管壁及平滑肌明显增厚、管腔狭窄;针刺组和艾灸组大鼠支气管管腔基本正常,可见少量炎性细胞的浸润,基底膜稍有增厚。模型组中IL-13、STAT6 mRNA的含量较空白组明显增高,差异具有统计学意义(P<0.05);经过艾灸或针刺治疗后IL-13、STAT6 mRNA的含量明显降低,差异具有统计学意义(P<0.05);针刺组和艾灸组比较,STAT6 mRNA差异具有统计学意义(P<0.05),IL-13差异无统计学意义(P>0.05)。结论:针灸能够降低哮喘模型大鼠血清中IL-13及肺组织中STAT6的阳性表达,进而改善气道炎症情况,这可能是针灸治疗哮喘的作用机理之一。Objective:To observe the effect of acupuncture and moxibustion on the expressions of IL-13 in serum and STAT6 in lung tissue of asthmatic model rats,and to explore the mechanism of needling and moxibustion on BL13,DU14 and BL12 in the treatment of asthma.Methods:40 SPF SD rats were randomly divided into the blank group,the model group,the moxibustion group and the acupuncture group,with 10 rats in each group.Asthma model was established by sensitization with ovalbumin(OVA)solution,and normal saline was used to replace ovalbumin in the blank group.The moxibustion group and the acupuncture group selected BL13,DU14,BL12 for moxibustion and acupuncture respectively;the blank group and the model group were not given any treatment,but with the same grip stimulation.After the last treatment,abdominal aortic blood was collected and the expression of IL-13 in serum was detected by ELISA;lung tissue was taken and the morphological changes of lung tissue were observed by HE staining;the expression of STAT6 mRNA in lung tissue was detected by RT-PCR.Results:HE staining showed that the bronchial lumen was round and regular,there was no obvious edema in bronchioles mucosa and there was no inflammatory cells infiltration in the bronchial lumen,alveolar cavity and interstitium in the blank group.In the model group,eosinophils and lymphocytes were the main inflammatory cells infiltrating into the lung tissue,the bronchial lumen was narrower,and the bronchial wall and smooth muscle were thicker than those in the blank group.The bronchial lumen was basically normal,only with a small amount of inflammatory cells infiltrating in vascular lumen,alveolar lumen and pulmonary interstitium,and the basement membrane was a slightly thick in the moxibustion group and in the acupuncture group.The contents of IL-13 and STAT6 mRNA in the model group were significantly higher than those in the blank group.After the intervention of moxibustion and acupuncture,the contents of IL-13 and STAT6 mRNA were significantly lowered(P<0.05).Compared to that

关 键 词：针刺 艾灸 哮喘 白介素-13 STAT6 mRNA 

分 类 号：R259[医药卫生—中西医结合] R245[医药卫生—中医内科学]