乌拉草提取液中木犀草素含量的测定及其抗菌性能  

Determination of luteolin content in carex meyeriana extract and its antibacterial properties

作　　者：王春红[1,2] 杨璐[1] 胡敏[1] 王晓云[1] 王利剑 WANG Chunhong;YANG Lu;HU Min;WANG Xiaoyun;WANG Lijian(School of Textile Science and Engineering, Tiangong University, Tianjin 300387, China;Key Laboratory of Advanced Textile Composite Materials, Tiangong University, Tianjin 300387, China)

机构地区：[1]天津工业大学纺织科学与工程学院,天津300387 [2]天津工业大学先进纺织复合材料重点实验室,天津300387

出　　处：《纺织学报》2021年第4期114-120,共7页Journal of Textile Research

基　　金：国家自然科学基金项目(11802205)。

摘　　要：为充分开发乌拉草的应用价值,探究其抗菌性能,采用水醇提取法制备了乌拉草提取液,研究了不同醇水比提取液的基本性能。利用紫外分光光度法和高效液相色谱法测试乌拉草提取液中抗菌物质木犀草素的含量,并对2种测试结果进行比较,采用体外抗菌法测试其抑菌率。结果表明:随着提取溶剂中水的比例的增加,提取液的电导率逐渐增大,pH值逐渐减小且均呈现酸性;紫外分光光度法及高效液相色谱法得出的木犀草素含量变化趋势一致,随着提取溶剂中水的比例的增大,木犀草素的含量均逐渐减少;不同醇水比的4种样品对大肠杆菌及金黄色葡萄球菌的抑菌率均大于70%,表明乌拉草提取液具有良好的抗菌性。In order to develop the application of carex meyeriana,and explore its antibacterial performance,the carex meyeriana extract was extracted by alcohol water mixture,and the basic performance of the extract with different alcohol water ratios were tested.The content of luteolin,an antibacterial substance,was tested by ultraviolet(UV)spectrophotometer and high performance liquid chromatography(HPLC)method,and the results of the two tests are compared.Its antibacterial rate was tested by in vitro antibacterial method.The results show that with the increase of the proportion of water in the extraction solvent,the conductivity of the extraction solution gradually increases and the pH value gradually decreases exhibiting acidity.The change trends of luteolin content detected by using UV spectrophotometer and HPLC method are the same.The content of luteolin decreases with the increase of the proportion of water in the extraction solvent,and the antibacterial rates of Escherichia coli and Staphylococcus aureus in the four samples with different alcohol water ratios were all higher than 70%,which indicates that the extract has good antibacterial properties.

关 键 词：乌拉草提取液 紫外分光光度法 高效液相色谱法 木犀草素 体外抗菌活性 抗菌性能 

分 类 号：TS101.4[轻工技术与工程—纺织工程] R284.1[轻工技术与工程—纺织科学与工程]