基于柠檬酸合成的新型可降解螺钉生物相容性和生物力学特性的研究  

Research on the biocompatibility and biomechanics of citrate-based biodegradable screws

作　　者：杨诚 李祯 廖坚文 樊仕才 杨健 白晓春[4] Yang Cheng;Li Zhen;Liao Jianwen;Fan Shicai;Yang Jian;Bai Xiaochun(Department of Orthopedic Surgery,The Third Affiliated Hospital of Southern Medical University,Academy of Orthopedics,Guangdong Province,Guangzhou,510630,China;Department of Anesthesiology,Zhongnan Hospital of Wuhan University,Wuhan 430071,China;Department of Biomedical Engineering,The Pennsylvania State University,University Park,PA 16802,USA;Department of Cell Biology,School of Basic Medical Science,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学第三附属医院创伤骨科,广东省骨科研究院,广州510630 [2]武汉大学中南医院麻醉科,武汉430071 [3]美国宾夕法尼亚大学材料与生物技术研究室,宾夕法尼亚州16802 [4]南方医科大学基础医学院细胞生物教研室,广州510515

出　　处：《中国临床解剖学杂志》2021年第2期182-186,共5页Chinese Journal of Clinical Anatomy

基　　金：广东省医学科研基金项目(A2017218)。

摘　　要：目的探讨聚柠檬酸复合纳米羟基磷灰石(POC-Click-HA)制作的新型生物可吸收松质骨螺钉治疗比格犬股骨外髁骨折的生物相容性及生物力学特性。方法健康成年比格犬9只,雌雄不限,体重8~11 kg,制备双侧股骨外髁骨折(AO分型33.B1型)模型。右侧采用POC-click-HA可吸收松质骨螺钉固定,作为实验组;左侧采用聚消旋乳酸PDLLA可吸收松质骨螺钉固定,作为对照组。术后观察实验动物一般情况,分别于术后4周、8周、12周处死3只动物,取材行大体观察、Lane-Sandhu组织学评分及生物力学分析。结果实验动物均存活至实验完成。大体观察两组骨折无移位,均于12周愈合,POCclick-HA组螺钉可见有新生骨组织从螺钉表面向内部生长。术后3个时间点对骨折部位进行LaneSandhu组织学评分,两组螺钉之间无统计学差异(P>0.05)。生物力学测试,4周POC-click-HA组钉-骨界面最大载荷低于PDLLA组(P<0.05),8周和12周两者无显著差异(P>0.05)。结论POC-click-HA松质骨螺钉具有良好的生物相容性,生物力学性能满足固定比格犬股骨外侧髁B1型骨折。Objective To evaluate the biocompatibility and biomechanics of citric acid copolymer-hydroxyapatite(POC-click-HA)screws in treatment of lateral femoral condyle fracture of canine.Methods Nine adult male Beagles(weighing,8-11 kg)were selected to prepare the models(AO classification type:B1)of bilateral lateral femoral condyle fracture.Right fracture was fixed with POC-click-HA absorbable cannulated screws as an experimental group and left fracture with Poly-DL-Lactic acid(PDLLA)screws as a control group.At 4,8,and 12 weeks after operation,general observation was done,Lane-Sandhu histologic grading,biomechanics were taken for observing fracture healing.Results All aniamls survived to the end of the experiment.General observations showed that no fracture displacement occurred and fracture healed at 12 weeks in the two groups.POC-click-HA screws degraded and new bone tissue was found from samples at 12 weeks.According to the Lane-Sandhu histologic grading,,there was no statistical difference between two groups in the screws at 4,8,and 12 weeks after operation(P>0.05).Biomechanics test showed that the maximum load at the nail-bone interface of the POC-click-HA group was lower than that of the PDLLA group at 4 weeks after operation(P<0.05).While there was no significant difference between the two groups at 8 weeks and 12 weeks(P>0.05).Conclusions Citric acid polymer composite hydroxyapatite(POC-clickHA)screw has good biocompatibility and biomechanics,which can be better utilized for Beagles’lateral femoral condyle B1 fractures.

关 键 词：可降解骨螺钉 股骨外髁骨折 柠檬酸 生物相容性 生物力学 

分 类 号：R318.08[医药卫生—生物医学工程]