暗纹东方鲀MyoD基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of MyoD Gene of Takifugu obscurus

作　　者：祁艳凤 尚凤芹 余云登 周金旭 王宁 王秀利 QI Yan-feng;SHANG Feng-qin;YU Yun-deng(College of Fisheries and Life Science,Dalian Ocean University,Dalian,Liaoning 116023)

机构地区：[1]大连海洋大学水产与生命学院,辽宁大连116023

出　　处：《安徽农业科学》2021年第8期89-93,共5页Journal of Anhui Agricultural Sciences

基　　金：国家海水鱼产业技术体系岗位科学家专项(CARS-47)。

摘　　要：提取暗纹东方鲀肌肉的总RNA,采用RT-PCR的方法来克隆暗纹东方鲀MyoD基因的cDNA序列,并通过生物信息学分析,对该基因所编码蛋白的理化性质、亲疏水性、亚细胞定位以及蛋白质结构等进行初步的分析和预测。结果显示,该基因编码区全长为846 bp,共编码了281个氨基酸,编码蛋白为亲水性非跨膜类蛋白,无信号肽结构,其亚细胞定位于细胞核中。系统进化树分析结果表明其与红鳍东方鲀同源性最高,亲缘关系最近。The total RNA of Takifugu obscurus muscle was extracted,and the cDNA sequence of MyoD gene of Takifugu obscurus was cloned by RT-PCR method.And through bioinformatics analysis,preliminary analysis and prediction of the physicochemical properties,hydrophilicity and hydrophobicity,subcellular localization and protein structure of the protein encoded by the gene were carried out.The results showed that the full length of the coding region of the gene was 846 bp,which encoded a total of 281 amino acids.The encoded protein was a hydrophilic non-transmembrane protein with no signal peptide structure,and its subcellular location was in the nucleus.The results of phylogenetic tree analysis showed that it had the highest homology with the redfin puffer and the closest genetic relationship.

关 键 词：暗纹东方鲀 MyoD基因 基因克隆 生物信息学 

分 类 号：S917.4[农业科学—水产科学]