水稻叶尖黄化突变体8272的候选基因筛选  

Screening of the Candidate Genes for a Rice Leaf Chlorosis Mutant 8272

作　　者：肖恩星 曾文静 曾彦达 张启军[1] 冯昊[2] XIAO En-xing;ZENG Wen-jing;ZENG Yan-da(Institute of Food Crops,Jiangsu Academy of Agricultural Science,Nanjing,Jiangsu 210014;College of Life Science,China West Normal University,Nanchong,Sichuan 637009)

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏南京210014 [2]西华师范大学生命科学学院,四川南充637009

出　　处：《安徽农业科学》2021年第8期94-98,共5页Journal of Anhui Agricultural Sciences

基　　金：国家转基因生物新品种培育重大专项(2016ZX08001004)。

摘　　要：对一份引自美国的水稻资源(编号为GSOR643131,2012年正季播种编号为128272,简写为8272)进行观察分析,发现该资源在较高温光条件下叶片发生不可逆的黄化,叶绿素含量明显下降;利用8272/9311杂交F2群体,明确了该性状受一个隐性核基因控制,并初步定位在水稻第4染色体SSR标记RM16931与RM16942之间,其遗传距离分别为0.8和3.3 cM。进一步设计引物和扩大F2分离群体,将该基因定位在RM16931与InDel标记ID42之间约117 kb的区间内;通过对8272和9311的重测序数据在引物区间进行SNP差异性筛选和候选基因的GO分析,结果表明,BGIOSGA 015140基因可能是引起8272黄化性状的候选基因。通过该研究可为解析水稻叶色变化机制奠定一定的基础。A rice resource 8272(GSOR643131)introduced from the United States,was found that the leaves were irreversibly yellowing under high temperature companied with high light intensity,its chlorophyll content was significantly reduced.F 2(8272/9311)population confirmed that this trait was regulated by a single recessive nuclear gene.Firstly,this gene was located between RM16931 and RM16942 on the chromosome 4 of rice,and with the genetic distance of 0.8 cM and 3.3 cM,respectively to the two markers.Secondly,it was located in a section about 117 kb between RM16931 and Indel marker ID42 using new primers and a bigger F2 population.Finally,re-sequencing data and SNP differential screening of 8272 and 9311 showed that BGIOSGA011540 gene might be the candidate gene.This study may lay a certain foundation for further resolution of the rice leaf color changing mechanism.

关 键 词：水稻 黄化突变体 基因定位 筛选 

分 类 号：S511[农业科学—作物学] S326