HPLC法测定不同产地川射干中6个活性成分的含量  

Simultaneous Determination of Six Active Constituents in Iridis Tectori Rhizoma from Different Habitats by HPLC

作　　者：郭焱 Guo Yan(Yancheng Food and Drug Supervision and Inspection Center,Jiangsu Yancheng 224000,China)

机构地区：[1]盐城市食品药品监督检验中心,江苏盐城224000

出　　处：《中国药师》2021年第4期759-762,共4页China Pharmacist

摘　　要：目的:比较不同产地川射干中射干苷、鸢尾甲苷A、鸢尾甲苷B、野鸢尾苷、野鸢尾黄素和次野鸢尾黄素6个活性成分的含量,为该药材资源的综合开发利用提供科学依据。方法:采用反相高效液相色谱法,色谱柱为Agilent Extend C18柱(250 mm×4.6 mm,5μm),以甲醇(A)-含0.3%甲基-β-环糊精的0.2%磷酸溶液(B)为流动相,梯度洗脱,流速1.0 ml·min-1,检测波长为265nm,柱温30℃。结果:在上述条件下,射干苷、鸢尾甲苷A、鸢尾甲苷B、野鸢尾苷、野鸢尾黄素和次野鸢尾黄素平均回收率分别为100.3%,98.8%,98.1%,101.0%,101.3%,100.8%,RSD分别为0.70%,1.53%,0.39%,1.57%,1.41%,0.98%(n=6);进样量分别在0.040~1.213μg,0.010~0.306μg,0.017~0.498μg,0.006~0.171μg,0.006~0.169μg,0.003~0.090μg范围内线性关系良好(r≥0.999 4);25批样品中6个成分的含量分别为5.923 5~43.514 4 mg·g-1,1.385 6~9.253 1 mg·g-1,3.526 2~16.028 5 mg·g-1,0.985 6~7.654 1 mg·g-1,2.965 6~12.354 1 mg·g-1,0.856 2~5.146 5 mg·g-1。结论:本试验所建立的方法可为川射干的质量控制提供科学的依据。Objective: To compare the contents of tectordin,iristetorin A,iristetetorin B,iridin,irigenin and irisflorentin in Iridis Tectori Rhizoma from different habitats,so as to provide scientific basis for the comprehensive development and utilization of this medicinal material resource. Methods: A RP-HPLC method was adopted. An Agilent Extend C18(250 mm×4.6 mm,5 μm) column was used. The mobile phase consisted of methanol-0.2% phosphate acid(adding 0.3% methyl-β-cyclodextrin) with gradient elution at a volume flow rate of 1.0 ml·min-1. The detection wavelength was set at 265 nm. The column temperature was at 30℃ . Results: Under the above conditions,the average recovery of tectordin,iristetorin A,iristetetorin B,iridin,irigenin and irisflorentin was 100.3%,98.8%,98.1%,101.0%,101.3% and 100.8%,with the RSDs of 0.70%,1.53%,0.39%,1.57%,1.41% and 0.98%(n = 6),respectively. The linear range of six components was 0.040-1.213 μg,0.010-0.306 μg,0.017-0.498 μg,0.006-0.171 μg,0.006-0.169μg and 0.003-0.090 μg,respectively(r≥0.999 4). The contents of 6 components in 25 samples were 5.923 5-43.514 4 mg·g-1,1.385 6-9.253 1 mg·g-1,3.526 2-16.028 5 mg·g-1,0.985 6-7.654 1 mg·g-1,2.965 6-12.354 1 mg·g-1 and 0.856 2-5.146 5 mg· g-1,respectively.Conclusion: The established method can provide scientific basis for the quality control of Iridis Tectori Rhizoma.

关 键 词：川射干 高效液相色谱法 含量测定 射干苷 鸢尾甲苷A 鸢尾甲苷B 野鸢尾苷 野鸢尾黄素 次野鸢尾黄素 

分 类 号：R927.2[医药卫生—药学]