加味四逆散对大鼠肝纤维化的改善作用  

Improved effect of Supplemental Sini San on hepatic fibrosis

作　　者：晁利芹[1] 王付[1] CHAO Li-qin;WANG Fu(Henan university of traditional Chinese medicine,Zhengzhou Henan,450046,China)

机构地区：[1]河南中医药大学中医药科学院,河南郑州450046

出　　处：《中西医结合肝病杂志》2021年第4期352-355,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：河南中医药大学博士基金(No.BSJJ2016-11);国家自然科学基金项目(No.81804196)。

摘　　要：目的:探讨加味四逆散对猪血清诱导的大鼠免疫损伤性肝纤维化的改善作用,为加味四逆散的科学应用提供依据。方法:采用腹腔注射未灭活猪血清联合保持激怒饥饿状态建立大鼠肝纤维化模型。造模结束后,连续56 d灌胃加味四逆散(7 mg/kg)及秋水仙碱(0.2 mg/kg)。观察各组大鼠中医症候指标、肝脏系数变化;测定血清肝功能、纤维化标志物的含量;肝组织TGF-β/Smad信号通路相关基因表达水平;MASSON染色观察肝组织病理学变化。结果:与模型组比较,阳性药物秋水仙碱组、加味四逆散组、预防组大鼠的肝脏系数,血清肝功能,纤维化标志物含量均显著下降(P<0.05);总蛋白、白蛋白、白球比水平均显著上升(P<0.05),血清球蛋白含量显著下降(P<0.05);体质量虽有增加,但与模型组无显著差异(P>0.05);TGF-β1、Smad3 mRNA表达均显著下降(P<0.05),Smad7 mRNA表达显著升高(P<0.05)。此外,加味四逆散组、秋水仙碱组、预防组之间结果无显著差异(P>0.05),但均未恢复到正常水平。肝脏病理组织切片结果显示加味四逆散组、阳性药物组、预防组肝脏病变程度明显减轻。结论:加味四逆散对猪血清联合激怒饥饿状态诱导大鼠肝纤维化具有改善疗效,且与秋水仙碱有相似的治疗效果。Objective:To investigate the effect of Supplemental Sini San on the immune induced liver fibrosis induced by porcine serum in rats,and to provide evidence for the scientific application of Supplemental Sini San.Methods:Rat liver fibrosis model was established by intraperitoneal injection of non inactivated pig serum in combination with maintaining irritability.After the end of modeling,the stagnation of sedum and colchicine(0.2 mg/kg)were administered intragastrically(7 mg/kg)for 56 days.The changes of TCM symptom index and liver coefficient of rats in each group were observed.The levels of serum liver function and fibrosis markers were determined.The expression levels of TGF-β/Smad signaling pathway-related genes in liver tissues were observed.The pathological changes of liver tissues were observed by MASSON staining.Results:Compared with the model group,the liver coefficient,serum liver function and fibrosis markers of the positive drug colchicine group,Supplemental Sini San group and prevention group were significantly decreased(P<0.05);total protein,white Protein and white spheres increased significantly(P<0.05),serum globulin content decreased significantly(P<0.05);although the body mass increased,there was no significant difference between the model and the model group(P>0.05);TGF-β1,Smad3 mRNA The expression was significantly decreased(P<0.05),and the expression of Smad7 mRNA was significantly increased(P<0.05).In addition,there was no significant difference between the Supplemental Sini San group,the colchicine group and the prevention group(P>0.05),but none of them returned to normal levels.The results of liver pathological biopsy showed that the degree of liver lesions in the Supplemental Sini San group,the positive drug group and the prevention group were significantly reduced.Conclusion:Supplemental Sini San has improved curative effect on liver fibrosis induced by porcine serum combined with irritability and starvation,and has similar therapeutic effect with colchicine.

关 键 词：加味四逆散 猪血清 肝纤维化 

分 类 号：R289.3[医药卫生—方剂学] R259[医药卫生—中药学]