舒芬太尼丙泊酚静脉复合麻醉对骨髓间充质干细胞移植治疗创伤性脑损伤大鼠的影响  

Effects of intravenous anesthesia with sufentanil combined with propofolin bone marrow mesenchymal stem cell transplantation on rats with traumatic brain injury

作　　者：武婕 陈燕[2] 杜晓宣[2] WU Jie;CHEN Yan;DU Xiao-xuan(Department of Anesthesiology, People′s Hospital of Longhua District, Guangdong Province, Shenzhen 518109, China;Department of Anesthesiology, the Sixth Affiliated Hospital of Xinjiang Medical University, Xinjiang, Urumqi 830002, China)

机构地区：[1]广东省深圳市龙华区人民医院麻醉科,广东深圳518109 [2]新疆乌鲁木齐市新疆医科大学第六附属医院麻醉科,新疆乌鲁木齐830002

出　　处：《河北医科大学学报》2021年第4期454-460,465,共8页Journal of Hebei Medical University

基　　金：新疆维吾尔自治区自然科学基金项目(2017D01C26)。

摘　　要：目的观察舒芬太尼丙泊酚静脉复合麻醉对骨髓间充质干细胞移植治疗创伤性脑损伤大鼠的影响。方法取3周龄幼鼠若干,体外分离、培养骨髓间充质干细胞(bone marrow mesenchymal stem cells)稳定传代3次备用。将45只受试大鼠随机分为对照组、干细胞治疗组及联合治疗组,每组15只。采用电子颅脑损伤仪(electric cortical contusion impactor,eCCI)建立颅脑损伤(traumatic brain injury,TBI)大鼠模型,造模完成后尾静脉给予干细胞治疗组干细胞悬液0.5 mL(1×105/mL),联合治疗组给予干细胞的同时尾静脉注射舒芬太尼0.5 g/kg和丙泊酚2.0 mg/kg,对照组给予等量生理盐水。神经功能评分用于判定治疗过程中大鼠神经损伤程度;HE染色观察大鼠脑组织病理学变化;干湿比重法、光谱扫描仪及酶联免疫吸附法(enzyme linked immunosorbene assay,ELISA)用于检测脑水含量、总钙含量及血清S100β含量变化;TUNEL法检测脑组织神经细胞凋亡情况,Western blot用于检测凋亡相关蛋白表达;qRT-PCR与免疫组化检测脑部炎性因子表达水平及分布。结果1:2传代后细胞呈放射状或旋涡状集落生长趋势,细胞形态随传代次数增加逐渐稳定均一,最终稳定生长为梭形成纤维细胞样。TBI造模结束后治疗3 d内,3组mNSS评分差异无统计学意义(P>0.05);治疗7 d后,联合治疗组mNSS评分显著低于对照组;治疗14 d、21 d后干细胞治疗组及联合治疗组评分均显著低于对照组,联合治疗组低于干细胞治疗组(P<0.05)。干细胞治疗组与联合治疗组海马组织较对照组病理学变化显著改善,且联合治疗组脑组织神经细胞密度显著恢复,海马区细胞排列整齐,细胞核完整且着色较浅,较干细胞治疗组治疗效果更佳。干细胞治疗组及联合治疗组脑组织水含量、总钙含量及血清S100β含量均低于对照组,联合治疗组低于干细胞治疗组(P<0.05)。干细胞治疗组、联合治疗组脑组织细胞凋�Objective To investigate the effect of intravenous anesthesia with propofol and sufentanil in bone marrow mesenchymal stem cell transplantation on the treatment of traumatic brain injury(TBI)in rats.Methods Bone marrow mesenchymal stem cells were isolated from young rats aged 3 weeks and cultured in vitro to 3 generations.A total of 45 healthy female SD rats were randomly divided into control group,stem cell treatment group and combination treatment group,with 15 rats in each group.TBI models were established by electric cortical contusion impactor(eCCI).After establishment of TBI modeling,the rats in stem cell treatment group were given 0.5 mL stem cell suspension(1×105/mL),the rats in combination treatment group were given stem cell suspension plus injection of 0.5 g/kg sufentanil and 2.0 mg/kg propofol,while the control group rats were given an equal volume of saline.Modified neurological function scores(mNSS)was performed to determine the degree of nerve damage in rats during treatment;morphological changes of brain tissue were observed by HE staining.The water content of brain,total calcium content and serum level of S100βwere measured by dry-and-wet gravity method,spectral scanner and enzyme linked immunosorbene assay.Neuronal apoptosis in hippocampal structural region was observed by TUNEL staining,western blot was used to detected the expression of apoptosis-related proteins,and quantitative real-time(qRT-PCR)and immunohistochemistry were used to detect the expression and distribution of inflammatory factors in brain tissues.Results After passage,the cells showed the trend of radial or vortex-like colony growth,and the cell morphology gradually became stable and uniform with the increase of passage times.Finally,the cells grew stably into spindle-like fibroblasts.Within 3 days after TBI modeling,there was no significant difference in mNSS score among the three groups(P>0.05);At 7 days after treatment,the mNSS score of the combination treatment group was significantly lower than that of the control group

关 键 词：脑损伤 舒芬太尼 丙泊酚 骨髓间充质干细胞 

分 类 号：R651.15[医药卫生—外科学]