硫酸-苯酚法测定六种牡丹叶中总多糖的含量  

Determination of Total Polysaccharide in Six Peony Leaves by Sulfuric Acid-Phenol Method

作　　者：牛晓方[1] 高一军 怀宝刚 尹二彦 马腾洲 李克明[3] 黄利敏 王培 侯明超 姜伟洲 NIU Xiaofang;GAO Yijun;HUAI Baogang;YIN Eryan;MA Tengzhou;LI Keming;HUANG Limin;WANG Pei;HOU Mingchao;JIANG Weizhou(Heze Municipal Hospital,Heze Shandong 274031;PingYi Hospital of Traditional Chinese Medicine,Pingyi Shandong 273300;Shandong Academy of Chinese Medicine,Jinan Shandong 250014;Weifang Hospital of Traditional Chinese Medicine,Weifang Shandong 261041)

机构地区：[1]菏泽市立医院,山东菏泽274031 [2]平邑县中医医院,山东平邑273300 [3]山东省中医药研究院,山东济南250014 [4]潍坊市中医医院,山东潍坊261041

出　　处：《山东农业工程学院学报》2021年第4期33-35,共3页The Journal of Shandong Agriculture and Engineering University

摘　　要：目的:利用硫酸-苯酚法测定六种不同产地牡丹叶中总多糖的含量,比较不同产地牡丹叶中总多糖的含量差异。方法:采用无水乙醇回流提取牡丹叶中的总多糖成分,所得样品加入苯酚、浓硫酸后采用紫外分光光度法测定总多糖的含量,以葡萄糖为对照品,波长为490nm。结果:6个产地的牡丹叶中总多糖的含量分别为1458.43、1400.79、1946.91、1243.37、1745.27、1345.78 ug/g。结论:所建立的方法能准确快速地对样品中的总多糖进行测定,产地不同,牡丹叶中总多糖含量存在差异。Objective:The content of total polysaccharide in peony leaves from six different habitats was determined by sulfuric acid-phenol method,and the difference of total polysaccharide content in peony leaves from different habitats was compared.Methods:The total polysaccharide in peony leaves was extracted by anhydrous ethanol reflux,The content of total polysaccharide was determined by UV spectrophotometry after adding phenol and concentrated sulfuric acid,With glucose as reference,490 nm.wavelength.Results:The contents of total polysaccharides in peony leaves from 6 regions were 1458.43、1400.79、1946.91、1243.37、1745.27、1345.78 ug/g.Conclusion:The established method can accurately and quickly determine the total polysaccharide in the sample.

关 键 词：牡丹叶 多糖 硫酸-苯酚法 

分 类 号：O621[理学—有机化学]