血浆相关生化指标的检测与奶牛妊检阴性的预警意义  

Detection of Plasma Biochemical Indexes and Risk Prediction of Negative Pregnancy Test in Dairy Cows

作　　者：孟德超 宋玉锡 曹浩 张桐宁 赵畅[1] 白云龙[1] 夏成[1] Meng Dechao;Song Yuxi;Cao Hao;Zhang Tongning;Zhao Chang;Bai Yunlong;Xia Cheng(College of Animal Science and Technology Heilongjiang Bayi Agricultural University Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases,Daqing 163319)

机构地区：[1]黑龙江八一农垦大学动物科技学院/黑龙江省牛病防控重点实验室,大庆163319

出　　处：《黑龙江八一农垦大学学报》2021年第2期33-39,共7页journal of heilongjiang bayi agricultural university

基　　金：国家自然基金面上项目(31772804);标准化养殖产业扶贫示范项目(ZY17C08);黑龙江垦区奶牛主要繁殖障碍疾病的综合防治关键技术研究与示范项目(HKKY190302)。

摘　　要：为了探究Presynch-Ovsynch(PO)程序处理前高产奶牛血浆生化指标对奶牛输精21 d后妊检阴性的风险预警。试验选择奶牛56头,在奶牛产后40 d采集血液用于检测血浆生化指标。在奶牛输精21 d后妊检并根据妊检结果分为P组(妊检阳性,N=26)和N组(妊检阴性,N=30)。应用独立样本t检验,斯皮尔曼相关分析,二元逻辑回归结合ROC分析。结果表明:N组奶牛血浆中HIS、P、BHBA、TG含量极显著高于P组(P<0.01);AST含量显著高于P组(P<0.05);E2、Glu、Se、Mg含量极显著低于P组(P<0.01)。血浆HIS、BHBA、AST、TG、P与21 d妊检阴性呈显著正相关(P<0.05);血浆E2、Glu、Se、Mg与21 d妊检阴性呈显著负相关(P<0.05);血浆E2<46.49 pmol·L^(-1)、Glu<3.59 mmol·L^(-1)、Se<0.58μg·L^(-1)为妊检阴性最佳预警值,因此血浆E2、Glu、Se对PO程序奶牛输精21 d后妊检阴性具有预警意义。To explore the risk prediction of negative pregnancy test after 21 days of insemination by plasma biochemical indexes of high-yield dairy cows before Presynch-Ovsynch(PO)program treatment,56 high-yield cows were selected.Blood was collected at 40 days postpartum to detect plasma biochemical indexes.According to the results of pregnancy test,the cows were divided into two groups:Group P(pregnancy test positive,N=26)and Group N(pregnancy test negative,N=30).Through independent sample t-test,Spearman correlation analysis,binary logistic regression and ROC analysis.The results showed that HIS,P,BHBA and TG in N group were extremely significantly higher than those in P group(P<0.01);AST in N group was significantly higher than those in P group(P<0.05);E2,Glu,Se,Mg in group N was significantly lower than those in group P(P<0.01).There was a significant positive correlation between HIS,BHBA,AST,TG,P and 21 d pregnancy test negative(P<0.05);There was a significant negative correlation between E2,Glu,Se,Mg and 21 d pregnancy test negative(P<0.05).E2<46.49 pmol·L^(-1),Glu<3.59 mmol·L^(-1),Se<0.58μg·L^(-1) was the best early warning value for negative pregnancy test.So E2,Glu and Se have early warning significance for negative pregnancy test of 21 days after insemination.

关 键 词：奶牛 生化检测 Presynch-Ovsynch程序 风险预警 

分 类 号：S823.89[农业科学—畜牧学]