分析临床基因扩增检验实验室的质量控制  

Analysis on the Quality Control of Clinical Gene Amplification Laboratory

作　　者：时超杰 朱一帆 李莎莎[1] 何敖林[1] SHI Chao-jie;ZHU Yi-fan;LI Sha-sha;HE Ao-lin(Clinical Experimental Research Center,Kunshan Hospital Affiliated to Jiangsu University,Kunshan,Jiangsu Province,215300 China)

机构地区：[1]江苏大学附属昆山医院临床实验研究中心,江苏昆山215300

出　　处：《中国卫生产业》2021年第1期190-192,共3页China Health Industry

摘　　要：聚合酶链反应(PCR)是一种体外核酸扩增技术,模拟自然DNA复制过程,通过重复高温变性、低温退火、中温延伸等简单的3个温度循环,将靶DNA扩增百万倍,显示极高的敏感性,因此在临床检测和科研中有着广泛的运用。做好PCR质量控制是保证检测结果准确性的关键步骤,该文就临床基因扩增检验实验室在实验前、中、后期中涉及的质量控制问题进行分析。Polymerase chain reaction(PCR)is an in vitro nucleic acid amplification technology that simulates the natural DNA replication process.It amplifies the target DNA by repeating 3 simple temperature cycles such as high temperature denaturation,low temperature annealing,and medium temperature extension,showing extremely high sensitivity,so it is widely used in clinical testing and scientific research.Performing PCR quality control is a key step to ensure the accuracy of test results.This article analyzes the quality control issues involved in clinical gene amplification testing laboratories before,during and after the experiment.

关 键 词：临床基因扩增检验实验室 PCR技术 质量控制 准确性 

分 类 号：R19[医药卫生—卫生事业管理]