改良硝酸银比色法测定车间空气中的硫化氢  

Determination of hydrogen sulfide in the air of workplace by improved silver nitrate method

作　　者：王育[1] WANG Yu(Tianning District Center for Disease Control and Prevention,Jiangsu Changzhou 213000,China)

机构地区：[1]常州市天宁区疾病预防控制中心,江苏常州213000

出　　处：《江苏预防医学》2021年第2期130-132,共3页Jiangsu Journal of Preventive Medicine

摘　　要：目的建立用分光光度法测定车间空气中硫化氢的方法。方法通过改变稳定剂,减少硫化银体系的散射,发现在420nm处产生吸收峰,该处的吸收光强度与硫化氢的含量呈良好的线性关系,据此建立测定车间空气中硫化氢的新方法,并优化最佳反应条件,建立标准曲线。结果硫化氢质量浓度在0.66~2μg/ml范围内线性关系良好,其线性回归方程为y=0.0314x+0.0247,相关系数r=0.9996,方法的检出限为0.2μg/ml。样品的加样回收率为90.5%~105.0%。结论通过OP(聚乙二醇辛基苯基醚)增稳,用分光光度法测定空气中的硫化氢更加准确、重现性好、简便、稳定。本法用于劳动卫生的车间空气检测可获得满意的结果。Objective To establish a novel method to determinate hydrogen sulfide in the air of workplace by spectorphotometry.Methods The scattering loss was reduced by changing stabilizer,resulting enhancement of absorbance intensity at 420 nm,demonstring agood linear relationship between the absorption light intensity and the content of hydrogen sulfide.A rapid,convenient novel spectrophotometry method to determine hydrogen sulfide was established,the reaction parameters were optimized,the standard curve was generated.Results The linear correlation between hydrogen sulfide mass concentration and absorption was good in the range of 0.66-2μg/ml.The linear regression equation was y=0.0314 x+0.0247,with correlation coefficient of 0.9996.The detection limit was 0.2μg/ml and the average spiked recovery was 90.5%-105.0%.Conclusion The determination of hydrogen sulfide in air by spectrophotometry is more accurate,reproducible,simple and stable by adding OP(polyethylene glycol octyl phenyl ether)for stabilization.The established method has been used successfully for the determination of H2 S in the air of workplace with satisfactory results.

关 键 词：硫化氢 硝酸银比色法 分光光度法 聚乙二醇辛基苯基醚 

分 类 号：R113[医药卫生—公共卫生与预防医学]