昆虫病原线虫共生菌NK水悬浮剂的研制  

Suspension concentrate of entomopathogenic nematode symbiotic bacteria NK

作　　者：廖春丽 杨溢 袁源 杜江南 樊星照 桑明博 朱菁 李冰冰 LIAO Chun-li;YANG Yi;YUAN Yuan;DU Jiang-nan;FAN Xing-zhao;SANG Ming-bo;ZHU-Jing;LI Bing-bing(College of Life Science&Engineering,Henan University of Urban Construction,Pingdingshan 467036,China;Henan Provincial Key Laboratory of Water Pollution Control and Rehabilitation Technology,Henan University of Urban Construction,Pingdingshan 467036,China)

机构地区：[1]河南城建学院生命科学与工程学院,河南平顶山467036 [2]河南城建学院河南省水体污染防治与修复重点实验室,河南平顶山467036

出　　处：《河南城建学院学报》2021年第1期80-86,共7页Journal of Henan University of Urban Construction

基　　金：河南城建学院青年骨干教师项目(G2016009)。

摘　　要：通过对昆虫病原线虫共生菌NK水悬浮剂所需各种助剂的筛选,辅以饲喂大蜡螟并对其防治效果进行评价,为该微生物资源的进一步实际应用打下基础。对各种助剂进行筛选,得出符合各项指标要求的共生菌NK水悬剂最优配方:细胞沉淀60%、山梨酸钾0.2%、尼泊金甲酯0.03%、脱氢醋酸钠0.2%、乳化剂OP-10为4%、乳化剂600#2%、全脂奶粉3%、黄原胶0.2%、丙三醇5%、有机硅0.6%和水24.77%。室内饲喂实验表明该制剂对3龄大蜡螟幼虫有良好的防治效果,原液和10倍、50倍、100倍稀释液对3龄大蜡螟幼虫7 d的防治效果分别是100%、88.89%、77.78%和61.11%,综合各方面因素考虑,推荐昆虫病原线虫共生菌NK水悬浮剂的使用浓度为10~50倍稀释液。The optimal formula of symbiotic bacteria NK suspension concentratea(SC)was obtained By screening of various additives which accorded with the requirements of various indexes.The formula was as follows:cell 60%,Potassium sorbate 0.2%,Methyl paraben 0.03%,Sodium Dehydroacetate 0.2%,OP-104%,Emulsifier 600#2%,milk powder 3%,Xanthan gum 0.2%,propanetriol 5%,Organic silicon 0.6%and water 24.77%.The experiment on indoor feeding showed that the 60%NK SC have a good control efficiency on 3rd-instar Galleria mellonella larva.The control efficiencies of 60%NK SC of 60%NK SC,10 times,50 times and 100 times dilution were 100%、88.89%、77.78%and 61.11%respectively in seven day.In the light of all aspects,It was recommended that the use concentration of the insect pathogenic nematode symbiotic bacteria NK SC was 10-50 times the diluent.

关 键 词：昆虫病原线虫 共生菌 水悬浮剂 大蜡螟 

分 类 号：S476[农业科学—农业昆虫与害虫防治]