黄芩素抑制高糖诱导的血管平滑肌细胞表型转化的实验研究  

Experimental study of baicalein inhibiting high glucose-induced phenotypic transformation of vascular smooth muscle cells

作　　者：周雯[1] 唐敏[2] 王咸寿 冯秋芳[2] 郑琳[2] 王青松[3] ZHOU Wen;TANG Min;WANG Xian-shou;FENG Qiu-fang;ZHENG Lin;WANG Qing-song(Department of Histology and Embryology,Hainan Medical College,Haikou 571199,China;Biotechnology Laboratory,Hainan Medical College,Haikou 571199,China;Department of Biochemistry and Molecular Biology,Hainan Medical College,Haikou 571199,China)

机构地区：[1]海南医学院组织胚胎学教研室,海南海口571199 [2]海南医学院生物技术专业实验室,海南海口571199 [3]海南医学院生物化学与分子生物学教研室,海南海口571199

出　　处：《海南医学院学报》2021年第8期561-565,571,共6页Journal of Hainan Medical University

基　　金：国家自然科学基金资助项目(82060153,81460043);海南省自然科学基金资助项目(820MS054,817141)。

摘　　要：目的:研究黄芩素在高糖诱导的血管平滑肌细胞表型转化中的作用。方法:大鼠血管平滑肌细胞实验分为正常对照组、黄芩素组、高糖组、高糖加黄芩素组共4组,荧光定量PCR分析α-SMA、SM22-α和OPN mRNA水平的改变,Western blot分析α-SMA、SM22-α和OPN蛋白水平的改变。结果:比较高糖处理组与高糖加黄芩素组,高糖处理组α-SMA mRNA为0.419±0.090,高糖加黄芩素组α-SMA mRNA为0.699±0.079,后者较前者升高66.8%;高糖处理组α-SMA蛋白为0.213±0.034,高糖加黄芩素组α-SMA蛋白为0.393±0.062,后者较前者升高84.5%;黄芩素能明显抑制高糖导致的α-SMA mRNA和蛋白表达的下调(P<0.05)。高糖处理组SM22-αmRNA为0.369±0.063,高糖加黄芩素组SM22-αmRNA为0.583±0.049,后者较前者升高58.0%;高糖处理组SM22-α蛋白为0.343±0.047,高糖加黄芩素组SM22-α蛋白为0.486±0.051,后者较前者升高41.7%;黄芩素能明显抑制高糖导致的SM22-αmRNA和蛋白表达的下调(P<0.05)。高糖处理组OPN mRNA为2.023±0.281,高糖加黄芩素组OPN mRNA 1.511±0.091,后者较前者降低25.3%;高糖处理组OPN蛋白为1.063±0.132,高糖加黄芩素组OPN蛋白为0.761±0.089,后者较前者降低28.4%;黄芩素能明显抑制高糖导致的OPN mRNA和蛋白表达的上调(P<0.05)。结论:黄芩素能明显抑制高糖导致血管平滑肌细胞从收缩型到合成型的表型转化。Objective:To investigate the role of baicalein in phenotypic transformation of vascular smooth muscle cells induced by high glucose.Methods:Rat vascular smooth muscle cell experiments were divided into the control group,the baicalein group,the high glucose group,the high glucose plus baicalein group.Real time quantitative PCR was used for mRNA analysis ofα-SMA,SM22-αand OPN,and Western blot was used for protein analysis ofα-SMA,SM22-αand OPN.Results:Comparing the high glucose group and the high glucose plus baicalein group,the level ofα-SMA mRNA in the high glucose group was(0.419±0.090),the level ofα-SMA mRNA in the high glucose plus baicalein group was(0.699±0.079),and the latter was 66.8%higher than the former.The level ofα-SMA protein in the high glucose group was(0.213±0.034),the level ofα-SMA protein in the high glucose plus baicalein group was(0.393±0.062),and the latter was 84.5%higher than the former.Baicalein could significantly inhibit the down-regulation ofα-SMA gene expression induced by high glucose(P<0.05).Comparing the high glucose group and the high glucose plus baicalein group,the level of SM22-αmRNA in the high glucose group was(0.369±0.063),the level of SM22-αmRNA in the high glucose plus baicalein group was(0.583±0.049),and the latter was 58.0%higher than the former.The level of SM22-αprotein in the high glucose group was(0.343±0.047),the level of SM22-αprotein in the high glucose plus baicalein group was(0.486±0.051),and the latter was 41.7%higher than the former.Baicalein could significantly inhibit the down-regulation of SM22-αgene expression induced by high glucose(P<0.05).Comparing the high glucose group and the high glucose plus baicalein group,the level of OPN mRNA in the high glucose group was(2.023±0.281),the level of OPN mRNA in the high glucose plus baicalein group was(1.511±0.091)and the latter was 25.3%lower than the former.The level of OPN protein in the high glucose group was(1.063±0.132),the level of OPN protein in the high glucose plus baicalein group

关 键 词：黄芩素 高糖 血管平滑肌细胞 表型转化 

分 类 号：R285.5[医药卫生—中药学]