1例猪链球菌5型感染者分离菌的病原学诊断、分型及相关基因的检测  

Etiological diagnosis,typing,and detection of related genes in isolates from a case of Streptococcus suis type 5 infection

作　　者：李曲文[1,2,3] 罗宏活 黄梦颖 谢芳钦[1,2] 林震宇 徐海滨[1,2] 翁顺太 LI Qu-wen;LUO Hong-huo;HUANG Meng-ying;XIE Fang-qin;LIN Zhen-yu;XU Hai-bin;WENG Shun-tai(Fujian Provincial Center for Disease Control and Prevention,Fuzhou 350001,China;Fujian Key Laboratory of Zoonosis,Fuzhou 350001,China;School of Public Health,Fujian Medical University,Fuzhou 350001,China;Sanming Center for Disease Control and Prevention,Sanming 365000,China)

机构地区：[1]福建省疾病预防控制中心,福州350001 [2]福建省人兽共患病研究重点实验室,福州350001 [3]福建医科大学公共卫生学院教学实习基地,福州350001 [4]三明市疾病预防控制中心,三明365000

出　　处：《中国人兽共患病学报》2021年第4期346-350,361,共6页Chinese Journal of Zoonoses

基　　金：福建省自然科学基金科技项目(No.2019J01093);福建省预防医学研究院建设(No.2019Y2001)。

摘　　要：目的对来自人感染猪链球菌患者血培养的分离株进行病原学分离鉴定,了解该菌株分子生物学特征,提供分子流行病原学依据。方法通过常规分离培养、生化鉴定、质谱检测分析、病原诊断基因及相关毒力基因检测和多位点序列分型技术(multi locus sequence typing,MLST)分析。结果菌株为猪链球菌血清型5型,毒力基因携带模式为mrp+/ef-/sly+/gdh+/gapdh+/fbps-/orf2-,MLST分型为ST373,该分离株对氯霉素和四环素耐药。结论猪链球菌血清型5型也可以引起人感染,可通过相关的分离培养鉴定以及相关的病原学基因进行快速诊断和流行病学研究。This study aimed to isolate and identify the pathogen from the blood culture of a person infected with Streptococcus suis 5,to understand the molecular biological characteristics of the strain,and to provide a basis for molecular epidemiology.We performed routine isolation and culture,biochemical identification,mass spectrometry analysis,detection of pathogen diagnostic genes and virulence-related genes,and multi locus sequence typing analysis.The strain was found to be Streptococcus suis serotype 5,the virulence gene carrying pattern was mrp+/ef-/sly+/gdh+/gapdh+/fbps-/orf2-,the MLST type was ST373,and the strain was resistant to chloramphenicol and tetracycline.Thus,Streptococcus suis serotype 5 can cause human infection and can be rapidly diagnosed through isolation,culture,and identification of related pathogenic genes.

关 键 词：猪链球菌 血清型5型 分子检测 

分 类 号：R378.1[医药卫生—病原生物学]