丹参酮ⅡA和丹酚酸B对大鼠血管外膜成纤维细胞转化的影响  

Effect of tanshinoneⅡA and salvianolic acid B on adventitia fibroblast transformation

作　　者：刘培[1] 姚东云[1] 邵铁梅[1] 安胜军[2] LIU Pei;YAO Dong-Yun;SHAO Tie-Mei(Bioreactor and Protein Drug Research and Development Center of Hebei Universities,Hebei Chemical and Pharmaceutical College,Shijiazhuang 050026,Hebei,China)

机构地区：[1]河北化工医药职业技术学院,河北省高校生物反应器与蛋白类药物开发应用技术研发中心,河北石家庄050026 [2]河北中医学院科研中心

出　　处：《中国老年学杂志》2021年第9期1889-1892,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81873230)。

摘　　要：目的探讨丹参酮ⅡA(TA)和丹酚酸(SA)B联合用药对成纤维细胞转化的影响。方法Transwell小室法检测TA和SAB对血管紧张素(Ang)Ⅱ诱导的细胞迁移的影响;Western印迹检测成纤维细胞α-平滑肌肌动蛋白(SMA)的表达;流式细胞术检测活性氧(ROS)水平的变化。结果TA和SAB组合(T8S5)对成纤维细胞的迁移能力有显著性抑制作用(P<0.01);T8S5对AngⅡ诱导的α-SMA的表达有显著抑制作用(P<0.01);T8S5能减少成纤维细胞内ROS的表达(P<0.01)。结论SAB和TA配伍组合能抑制AngⅡ诱导的大鼠血管外膜成纤维细胞的迁移和转化。Objective To explore the effects of tanshinoneⅡA(TA)and salvianolic acid(SA)B in combination on the differentiation of rat vascular adventitial fibroblasts.Methods The effect of TA and SAB in combination on the migration of adventitial fibroblast induced by Angiotensinogen(Ang)Ⅱwere evaluated by Transwell,and the effect of TA and SAB in combination on the expression ofα-smooth muscle actin(SMA)was detected by Western blot.The reactive oxygen species(ROS)level was detected by flow cytometry.Results The results of Transwell showed that TA and SAB in combination(T8S5)inhibited the migration of fibroblasts(P<0.01).The results of Western blot showed that T8S5 significantly inhibited the expression ofα-SMA induced by AngⅡ(P<0.01).Flow cytometry showed that T8S5 decreased the expression of ROS(P<0.01).Conclusions TA and SAB in combination could inhibit AngⅡ-induced migration and transformation of rat adventitial fibroblasts.

关 键 词：丹参酮ⅡA 丹酚酸B 迁移 转化 活性氧 

分 类 号：R2-33[医药卫生—中医学] R-332