白细胞介素-38对ApoE^(-/-)小鼠动脉粥样硬化作用及机制研究  

Effects of IL-38 on atherosclerosis in ApoE^(-/-) mice and their mechanisms

作　　者：魏辉[1,2] 李慧 余国华[1] 董素娟 朱卫芳[1] WEI Hui;LI Hui;YU Guohua;DONG Sujuan;ZHU Weifang(The First People Hospital of Tianmen,Tianmen 431700,China;Hubei Province Key Laboratory of Occupational Hazard Identification and Control,Wuhan University of Science and Technology,Wuhan 430065,China;不详)

机构地区：[1]天门市第一人民医院,湖北天门431700 [2]武汉科技大学职业危害识别与控制湖北省重点实验室,武汉430065

出　　处：《实用医学杂志》2021年第7期858-862,共5页The Journal of Practical Medicine

基　　金：湖北省卫生健康委联合基金项目(编号:WJ2019H210)。

摘　　要：目的通过构建动脉粥样硬化模型,探讨白细胞介素-38(IL-38)对载脂蛋白E基因敲除(ApoE^(-/-))小鼠动脉粥样硬化形成的影响及机制。方法 6周龄雄性ApoE^(-/-)小鼠随机分为对照组(腹腔注射磷酸盐缓冲液)和外源重组IL-38组(腹腔注射rIL-38),给予12周西方饮食饲料喂养,建立动脉粥样硬化模型。给药结束后,通过油红O染色观察小鼠动脉粥样硬化程度,检测血清TC、TG水平;利用酶联免疫法检测血浆IFN-γ、IL-17、IL-10及TGF-β水平;利用流式细胞仪检测脾脏Th1、Th17、Treg及M2比例指标;观察斑块的稳定性等指标。结果与对照组相比,rIL-38组主动脉窦和全主动脉斑块面积显著减少(P<0.01),且斑块内胶原纤维、平滑肌细胞显著增加(P<0.05),而CD4~+T细胞、巨噬细胞、MMP-2和MMP-9显著减少(P<0.05)。rIL-38组脾脏Th1、Th17细胞比例显著减少,M2、Treg显著增加(P<0.05)。血浆IFN-γ、IL-17水平降低,IL-10、TGF-β水平升高(P<0.05)。结论外源重组rIL-38能抑制动脉粥样硬化进程和促进斑块稳定。Objective To explore the effects of interleukin 38(IL-38)on the formation of atherosclerosis and their and mechanisms in ApoE^(-/-)mice by constructing an atherosclerosis model.Methods 6-week-old male ApoE^(-/-)mice were randomly divided into two groups.They were fed with Western-style diets for 15 weeks.The mice in the control group were injected intraperitoneally with phosphate buffered solution and so were those in the model group injected with exogenous recombinant IL-38.After administration,the plaque areas were detected by oil red O.The plaque stability was observed;Th1,Th17,and Treg and M2 ratio in the spleen,plasma levels of IFN-γ,IL-17,IL-10 and TGF-β,and serum levels of TC and TG were detected.Results As compared with the control group,the plaque areas in aortic sinus and entire aorta in the rIL-38 group were significantly reduced(P<0.01)and collagen fibers and smooth muscle cells in the plaque increased significantly(P<0.05),while CD4+T cells,macrophages,MMP-2,and MMP-9 were significantly reduced(P<0.05);the splenic ratio of Th1 to Th17 cells in the rIL-38 group was significantly reduced;M2 and Treg were significantly increased;plasma levels of IFN-γand IL-17 decreased but levels of IL-10 and TGF-βincreased(P<0.05).Conclusions Exogenous rIL-38 can inhibit the process of atherosclerosis and promote the stability of plaque.

关 键 词：动脉粥样硬化 白细胞介素38 炎症因子 抗炎因子 

分 类 号：R541.4[医药卫生—心血管疾病]