神经调节蛋白1/转化生长因子β1对血管平滑肌细胞骨架的作用  

Effect of NRG-1/TGF-β1 on cytoskeleton of vascular smooth muscle

作　　者：李海涛[1] 林劲[1] 韩丽珍[1] 王裕岱[1] 滕丽峰 LI Haitao;LIN Jin;HAN Lizhen;WANG Yudai;TENG Lifeng(Department of Cardiology,Hainan Provincial People's Hospital(Hainan Hospital Affiliated to Hainan Medical College),Haikou 570311,China)

机构地区：[1]海南省人民医院(海南医学院附属海南医院)心血管内科,海口570311

出　　处：《实用医学杂志》2021年第7期863-868,共6页The Journal of Practical Medicine

基　　金：海南省高层次人才基金(编号:2019RC368)。

摘　　要：目的探讨神经调节蛋白1/转化生长因子β1(NRG-1/TGF-β1)对血管平滑肌细胞骨架的作用。方法取小鼠血管组织,采用免疫荧光法对NRG-1干预后细胞收缩功能变化进行分析,采用鬼笔环肽、免疫荧光共定位分析NRG-1/TGF-β1表达,采用GST融合蛋白沉降技术检测分析NRG-1-ICD与α-肌动蛋白(α-SMA)的共位性。结果 TGF-β1在人主动脉血管平滑肌细胞中可上调NRG-1表达,经免疫荧光染色可发现平滑肌细胞标志蛋白α-SMA与NRG-1存在共位性;经RT-PCR及Western blot检测分析发现,在大鼠平滑肌细胞、人平滑肌细胞以及人内皮细胞中均存在NRG-1的mRNA表达。结论在转录和翻译水平上TGF-β1可上调NRG-1的表达;在细胞收缩中,TGF-β1对于α-SMA与NRG-1-ICD之间的表达可以起到促进作用。Objective To investigate the effect of neuregulin 1/transforming growth factorβ1(NRG-1/TGF-β1)on the cytoskeleton of vascular smooth muscle.Methods Blood vessel tissues of mice were taken,and the changes of cell contractile function after NRG-1 intervention were analyzed by immunofluorescence method,and the expression of NRG-1/TGF-β1 was analyzed by phalloidin and immunofluorescence co-localization,and the GST fusion protein was used for sedimentation.Technical detection and analysis of the co-location of NRG-1-ICD andα-actin(α-SMA)were performed.Results TGF-β1 could up-regulate the expression of NRG-1 in human aortic vascular smooth muscle cells.Immunofluorescence staining showed that the smooth muscle cell marker proteinα-SMA and NRG-1 were co-located;RT-PCR and Western blot found that NRG-1 mRNA expression existed in rat smooth muscle cells,human smooth muscle cells and human endothelial cells.Conclusion TGF-β1 can up-regulate the expression of NRG-1 at the transcription and translation levels;in cell contraction,TGF-β1 can promote the expression ofα-SMA and NRG-1-ICD.

关 键 词：血管平滑肌细胞 Α-肌动蛋白 神经调节蛋白1 转化生长因子Β1 血小板衍生生长因子 胞内结构域 

分 类 号：R543[医药卫生—心血管疾病]