2014年湖南省C亚属人腺病毒1株临床分离株的全基因组特征分析  

Analysis of whole genome characteristics of 1 clinical subgenus C human adenovirus strain isolated from Hunan province in 2014

作　　者：马若群 向星宇[2] 张红[2] 李芳彩[2] 黄超洋[2] 崔爱利[1] 张燕[1] 许文波[1] 毛乃颖[1] 朱贞[1] Ma Ruoqun;Xiang Xingyu;Zhang Hong;Li Fangcai;Huang chaoyang;Cui Ailii;Zhang Yan;Xu Wenbo;Mao Naiying;Zhu Zhen(NHC Key Laboratory of Medical Virology and Viral Diseases,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China;Hunan Center for Disease Control and Prevention,Changsha 410005,China)

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,国家卫生健康委员会医学病毒和病毒病重点实验室,北京102206 [2]湖南省疾病预防控制中心,长沙410005

出　　处：《中华实验和临床病毒学杂志》2021年第1期49-56,共8页Chinese Journal of Experimental and Clinical Virology

基　　金：国家重点研发计划(2018YFC1200602,2017YFC1200303);"十三五"国家科技重大专项(2017ZX10104001-002,2018ZX10713002,2018ZX10713001-003)。

摘　　要：目的了解2014年湖南省C亚属人腺病毒(HAdV-C)分离株的基因特征。方法2014年从湖南省长沙市严重急性呼吸道感染儿童病例咽拭子标本中分离到一株HAdV-C病毒株(Hunan2014-s024),分段扩增目的基因片段,拼接后获得全基因组序列,同时下载GenBank数据库中国内外流行的HAdV-C代表株全基因组序列构建HAdV-C数据库,使用生物信息学软件进行全基因组序列特征分析。结果Hunan2014-s024株与2006年山西省健康儿童粪便标本中分离到的HAdV-C病毒株(MK041244-CHN-2006)的同源性最高,并以该病毒基因组元件为主要骨架,在penton base基因上交换了2012年湖南省急性下呼吸道感染患儿分离株(MK041246-CHN-2012)的基因片段。结论2014湖南株(Hunan2014-s024)为2006年山西省病毒株(MK041244-CHN-2006)与2012年湖南株(MK041246-CHN-2012)的重组病毒,由于其致病性可能发生了改变,因此有必要加强HAdV-C的分子流行病学监测,以了解其潜在疾病负担和公共卫生意义。Objective To understand the genetic characteristics of subgenus C human adenovirus(HAdV-C)strain isolated in Hunan in 2014.Methods An HAdV-C strain named Hunan2014-s024 was isolated from throat swab collected from a child with severe acute respiratory infection in Changsha city of Hunan province in 2014.The whole genome sequence(WGS)was obtained by segmented amplification and sequence splicing.Database of HAdV-C was constructed with the strain from this study and the HAdV-C representative strains from GenBank.The whole genome characteristics was analyzed by using bioinformatics software.Results Strain Hunan2014-s024 has the highest homology with the HAdV-C strain(MK041244-CHN-2006)isolated from stool sample of a healthy child in Shanxi province in 2006,and the genome of strain MK041244-CHN-2006 was used as the backbone,and penton based gene was replaced by gene fragment from MK041246-chn-2012 strain isolated from a child with acute lower respiratory tract infection in Hunan province in 2012.Conclusions Our result indicated that 2014 Hunan strain(Hunan2014-s024)is a recombinant virus of 2006 Shanxi strain(MK041244-CHN-2006)and 2012 Hunan strain(MK041246-CHN-2012).Due to the change of pathogenicity,it is necessary to strengthen the molecular epidemiological surveillance of HAdV-C to understand its potential disease burden and public health significance.

关 键 词：湖南省 C亚属人腺病毒 基因特征 

分 类 号：R446.5[医药卫生—诊断学] R725.6[医药卫生—临床医学]