Aloin attenuates chronic constriction injury-induced neuropathic pain in rats by inhibiting inflammatory cytokines and oxidative stress  

作　　者：Aarti S.Kale Avinash R.Wadkar Umesh B.Mahajan Lalit A.Birari Sateesh Belemkar Sameer N.Goyal Shreesh Ojha Sanjay J.Surana Chandragouda R.Patil Kalpesh R.Patil 

机构地区：[1]Department of Pharmacology,R.C.Patel Institute of Pharmaceutical Education and Research,Shirpur-425405,Dist.Dhule,Maharashtra,India [2]School of Pharmacy&Technology Management,SVKM's NMIMS,MPTP,Shirpur-425405,Dist-Dhule,Maharashtra,India [3]Shri Vile Parle Kelavani Mandal's Institute of Pharmacy,Dhule-424001,Maharashtra,India [4]Department of Pharmacology and Therapeutics,College of Medicine and Health Sciences,United Arab Emirates University,P.O.Box 17666,Al Ain,Abu Dhabi,UAE [5]Department of Pharmacognosy,R.C.Patel Institute of Pharmaceutical Education and Research,Shirpur-425405,Dist.Dhule,Maharashtra,India

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第5期205-213,共9页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To investigate the effect of aloin against chronic constriction injury(CCI)-induced neuropathic pain in rats.Methods:Rats were randomly divided into 7 groups:GroupⅠ(normal control),GroupⅡ(sham-operated),GroupⅢ(CCI control)and GroupⅣ,Ⅴ,Ⅵ,andⅦ,which underwent CCI surgery and then were administered with aloin(5 mg/kg,p.o.;25 mg/kg,p.o.;125 mg/kg,p.o.)and gabapentin(50 mg/kg,p.o.),respectively for 14 days.Peripheral neuropathy was induced by silk ligatures(4-0)loosely placed around the sciatic nerve.Nociceptive thresholds against mechanical stimuli(Von-Frey filaments)and thermal stimuli(12℃and 40℃)were measured at midplantar paw region ipsilateral to the compressed nerve on day-3,7,11,and 14.The concentration of cytokines including tumor necrosis factor-α(TNF-α),interleukin-6,and interleukin-1βwas estimated at day-7.At day 14,motor nerve conduction velocity was determined under urethane anesthesia(1.25 g/kg).Oxidative stress parameters(malondiadehyde,glutathione,catalase,and superoxide dismutase)were estimated in sciatic nerve homogenates at day 14.Representative nerve samples were processed for histological investigations.Results:Aloin significantly reduced CCI-induced mechanical and thermal allodynia.It also improved motor nerve conduction velocity and decreased oxidative stress in nerve tissues.In addition,it decreased pro-inflammatory cytokine levels and restored the histoarchitecture of compressed sciatic nerve.Conclusions:Aloin mitigates CCI-induced neuropathic pain in rats by inhibiting oxidative stress and pro-inflammatory cytokines in the afflicted sciatic nerve.

关 键 词：ALOIN Chronic constriction injury ANTIOXIDANT Neuropathic pain 

分 类 号：R285.5[医药卫生—中药学]