Argemone mexicana extract alleviates gastrointestinal disorders by stimulating muscarinic receptors and blocking voltage-gated L-type calcium channels  

作　　者：Rabia Iqbal Irfan Hamid Khalid Hussain Janbaz Muhammad Furqan Akhtar Ammara Saleem Ali Sharif Sohaib Peerzada Bushra Akhtar Kashif Sohail Sajid Ali 

机构地区：[1]Faculty of Pharmacy,The University of Lahore,Lahore,Pakistan [2]Cadson College of Pharmacy,University of the Punjab,Lahore,Pakistan [3]Department of Pharmacology,Bahauddin Zakariya University,Multan,Pakistan [4]Riphah Institute of Pharmaceutical Sciences,Riphah International University,Lahore Campus,Lahore,Pakistan [5]Department of Pharmacology,Faculty of Pharmaceutical Sciences,Government College University Faisalabad,Faisalabad,Pakistan [6]Department of Pharmacology,Faculty of Pharmaceutical and Allied health Sciences,Lahore College for Women University,Lahore,Pakistan [7]Institute of Pharmacy,Physiology and Pharmacology,University of Agriculture,Faisalabad,Pakistan [8]Akson College of Pharmacy,Mirpur University of Science and Technology,Mirpur,AJK,Pakistan

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2021年第5期214-221,共8页亚太热带生物医学杂志（英文版）

摘　　要：Objective:To investigate the pharmacological potential of Argemone mexicana in treating constipation and emesis by using in vitro and in vivo models.Methods:The spasmogenic and spasmolytic effects were evaluated on isolated rabbit jejunum fragments loaded in a tissue organ bath.The response was recorded with an isotonic transducer attached with Power Lab Data Acquisition System.The laxative and antiemetic activities were assessed in BALB-c mice and poultry chicks challenged with carbamylcholine and copper sulphate stimulated emesis,respectively.Results:The total phenolic and total flavonoids contents of the extract were(267.75±5.77)mg GAE/g and(73.86±6.01)mg QE/g,respectively.Argemone mexicana extract exerted spasmogenic effect on isolated rabbit jejunum segments with an EC_(50)value of 0.016 mg/m L,which was blocked by atropine(0.3μM).Argemone mexicana extract exerted spasmolytic effect in atropine treated jejunum fragments with an EC_(50)value of 2.185 mg/mL.Furthermore,Argemone mexicana extract relaxed potassium(80 mM)-induced contractions(EC_(50):9.07 mg/mL),similar to a standard drug verapamil.The calcium channel blocker activity was confirmed by a rightward shift of concentration-response curve of calcium in the presence of Argemone mexicana extract(1-5 mg/mL)and verapamil(0.1-1μM).In addition,the extract increased the distance travelled by a charcoal in the gastrointestinal tract and exhibited antiemetic effect on copper sulphate induced emesis in chicks.Conclusions:Argemone mexicana shows cholinergic agonist and calcium channel blocker activities,as well as antiemetic effect.It may be used as a potential agent for treating gastrointestinal disorders.

关 键 词：Argemone mexicana Muscarinic agonist Calcium channel blocker CONSTIPATION EMESIS 

分 类 号：R965[医药卫生—药理学]