miRNA-27α调控PPARγ/ApoA5通路对大鼠激素性股骨坏死的影响研究  

Effect of miRNA-27αregulating PPARγ/ApoA5 pathway on hormonal femoral necrosis in rats

作　　者：黄涛[1] 崔泳[1] Huang Tao;Cui Yong(Department of Orthopedics,the Fifth Affiliated Hospital,Xinjiang Medical University,Urumqi 830000,China)

机构地区：[1]新疆医科大学第五附属医院骨科,新疆乌鲁木齐830000

出　　处：《实用骨科杂志》2021年第4期330-334,共5页Journal of Practical Orthopaedics

基　　金：新疆维吾尔自治区自然科学基金(2017D01C276)。

摘　　要：目的探究甲泼尼龙诱导的激素性股骨头坏死模型中miRNA-27α调控过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptor,PPARγ)/载脂蛋白A5(apolipoprotein A5,ApoA5)通路对大鼠激素性股骨坏死的影响。方法30只SPF级SD雌性大鼠根据随机数字法分为5组,每组6只,其中1组为空白对照组,4组为模型组。采用甲泼尼龙诱导构建体外股骨头坏死模型。慢病毒转染骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)后干细胞注射,免疫组化分析骨形态,实时荧光定量聚合酶链式反应(real time fluorescent quantitative polymerase chain reaction,qRT-PCR)分析基因表达量变化,噻唑蓝(methyl thiazolyl tetrazolium,MTT)法检测细胞增殖与活力。结果激素诱导大鼠股骨头切片显示骨小梁出现稀疏、断裂、排列紊乱,骨小梁的面积显著减少,miRNA-27α的含量显著降低(P<0.05),PPARγ和ApoA5 mRNA表达水平均显著升高(P<0.05);PPARγ和ApoA5与miRNA-27α呈现负相关,相关系数分别为0.725和0.683;注射甲泼尼龙和miRNA-27αmimics组,PPARγ和ApoA5 mRNA表达水平均显著降低(P<0.05);添加miRNA-27αmimics组细胞增殖水平显著高于miRNA-27αinhibitor组。模型组软骨细胞的存活率都显著降低,但同时添加miRNA-27αinhibitor组产生了更大的抑制作用。结论miRNA-27α通过抑制PPARγ/ApoA5的表达延缓了激素性股骨头坏死的进程。Objective To investigate the effect of miRNA-27αregulating peroxisome proliferator-activated receptor(PPARγ)/apolipoprotein A5(ApoA5)pathway on steroid-induced femoral head necrosis in rats induced by methylprednisolone.Methods Thirty female SPF-grade SD rats were divided into 5 groups according to the random number method,with 6 in each group.1 group was a blank control group;4 groups were model groups.An in vitro model of femoral head necrosis was induced by methylprednisolone.Immunohistochemical analysis of bone morphology,qRT-PCR analysis of gene expression changes,and MTT method were used to detect cell proliferation and viability.Results Hormone-induced rat femoral head sections showed sparse,broken,and disordered bone trabeculae,significantly reduced the area of bone trabeculae,and significantly reduced the content of miRNA-27α(P<0.05).The levels of PPARγand ApoA5 mRNA expression were significant increased(P<0.05).PPARγand ApoA5 showed a negative correlation with miRNA-27α,with correlation coefficients of 0.725 and 0.683,respectively.In the injection group of methylprednisolone and miRNA-27αmimics,the levels of PPARγand ApoA5 mRNA significantly decreased(P<0.05);the proliferation of miRNA-27αmimics group was significantly higher than that of miRNA-27αinhibitor group.The chondrocyte survival rate in the model group was significantly reduced,but the miRNA-27αinhibitor group also produced a greater inhibitory effect.Conclusion miRNA-27αcan delay the progress of hormonal femoral head necrosis by inhibiting the expression of PPARγ/ApoA5.

关 键 词：miRNA-27α 过氧化物酶体增殖物激活受体Γ 载脂蛋白A5 激素性股骨坏死 

分 类 号：R681.8[医药卫生—骨科学]