通腑泄热法对兔肝部分切除术后肝功能的影响  

Effect of Tongfu Xiere method on liver function after partial hepatectomy in rabbits

作　　者：袁辉 翟淑萍 苑伟 张旭[1] 毛本亮 曾宏兴 王百林 Yuan Hui;Zhai Shuping;Yuan Wei;Zhang Xu;Mao Benliang;Zeng Hongxing;Wang Bailin(Guizhou Medical University,Guiyang 550004,China;Department of General Surgery,Guangzhou Red Cross Hospital,Guangzhou 510220,China;Graduate School of School of Medicine,Jinan University,Guangzhou 510632,China)

机构地区：[1]贵州医科大学,贵阳550004 [2]广州市红十字会医院普通外科,510220 [3]暨南大学医学院研究生院,广州510632

出　　处：《中华实验外科杂志》2021年第3期458-461,共4页Chinese Journal of Experimental Surgery

基　　金：广州市中医药和中西医结合科技项目(20202A011010)。

摘　　要：目的观察通腑泄热法中药对兔肝部分切除术后肝功能的影响。方法选用新西兰白兔25只,以随机数字表法随机分为3组,即中药组(TCM组,n=10),对照组(Control组,n=10),假手术组(Sham组,n=5),制备兔肝部分切除模型20例和假手术模型(切开腹壁后关腹)5例,分别于术前、术后第1天、术后第3天、术后第5天,检测各组兔血清中过氧化氢酶(CAT)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(ALB)的含量水平。多组间样本比较采用单因素方差分析,组间两样本比较采用独立样本t检验。结果术前、术后第1天、术后第3天、术后第5天,中药组CAT的含量分别为(6.34±0.61)、(4.44±0.72)、(5.09±0.36)、(6.18±0.62)U/ml;对照组CAT的含量分别为(6.08±0.80)、(4.48±0.72)、(4.64±0.49)、(5.05±0.39)U/ml;假手术组CAT的含量分别为(6.34±0.64)、(6.31±0.52)、(6.33±0.42)、(6.36±0.31)U/ml。中药组ALT的含量分别为(25.67±2.97)、(38.65±3.14)、(33.30±2.33)、(26.95±1.74)U/L;对照组ALT的含量分别为(26.04±2.13)、(36.02±4.08)、(35.70±1.96)、(32.55±4.68)U/L;假手术组ALT的含量分别为(25.13±2.19)、(25.22±2.87)、(25.06±2.21)、(24.04±1.39)U/L。中药组AST的含量分别为(18.61±1.79)、(29.31±2.78)、(25.95±3.33)、(19.76±1.91)U/L;对照组AST的含量分别为(17.32±2.21)、(28.00±3.76)、(27.29±2.78)、(26.05±2.41)U/L;假手术组AST的含量分别为(18.27±2.15)、(18.92±2.41)、(18.87±1.89)、(18.54±2.28)U/L。中药组ALB的含量分别为(51.31±5.43)、(41.08±1.96)、(45.40±2.43)、(48.74±3.26)g/L;对照组ALB的含量分别为(52.14±4.36)、(42.99±4.16)、(45.33±3.71)、(43.74±2.95)g/L;假手术组ALB的含量分别为(50.05±4.23)、(51.18±4.11)、(51.10±2.93)、(51.50±3.34)g/L。CAT、ALT、AST、ALB的含量变化,术前各组间差异无统计学意义(CAT,t=0.815、-0.003、-0.633;ALT,t=-0.322、0.357、0.775;AST,t=1.443、0.325、-0.798;ALB,t=-0.377、0.451、0.881,P值均>0.05)。术后Objective To observe the effect of Tongfu Xiere method on liver function after partial hepatectomy in rabbits.Methods Totally,25 New Zealand white rabbits were selected and randomly divided into 3 groups using the number table method:Chinese medicine group(TCM group,n=10),control group(n=10),sham operation group(Sham group,n=5).A total of 20 cases of rabbit partial hepatectomy model and 5 casesof sham operation model(to cut the abdominal wall and close the abdomen)were prepared respectively.Before and 1,3 and 5 dafter operation,serum catalase(CAT),alanine aminotransferase(ALT),aspartate aminotransferase(AST),albumin(propagated)levels were determined.Using SPSS 20.0 statistical software analysis,measurement data are expressed as mean±standard deviation(Mean±SD),sample comparison among multiple groups was performed by one-way analysis of variance,and two samples between groups were compared by independent sample t test.Results Before,and 1,3 and 5 d after operation,CAT content in each group was as follows:TCM group[(6.34±0.61),(4.44±0.72),(5.09±0.36),(6.18±0.62)U/ml];control group[(6.08±0.80),(4.48±0.72),(4.64±0.49),(5.05±0.39)U/ml];sham operation group[(6.34±0.64),(6.31±0.52),(6.33±0.42),(6.36±0.31)U/ml].ALT content in each group was as follows:TCM group[(25.67±2.97),(38.65±3.14),(33.30±2.33),(26.95±1.74)U/L];control group[(26.04±2.13),(36.02±4.08),(35.70±1.96),(32.55±4.68)U/L];sham operation group[(25.13±2.19),(25.22±2.87),(25.06±2.21),(24.04±1.39)U/L].AST content in each group was as follows:TCM group[(18.61±1.79),(29.31±2.78),(25.95±3.33),(19.76±1.91)U/L];control group[(17.32±2.21),(28.00±3.76),(27.29±2.78),(26.05±2.41)U/L];sham operation group[(18.27±2.15),(18.92±2.41),(18.87±1.89),(18.54±2.28)U/L].ALB content in each group was as follows:TCM group[(51.31±5.43),(41.08±1.96),(45.40±2.43),(48.74±3.26)g/L];control group[(52.14±4.36),(42.99±4.16),(45.33±3.71),(43.74±2.95)g/L];sham operation group[(50.05±4.23),(51.18±4.11),(51.10±2.93),(51.50±3.34)g/L].There was no

关 键 词：肝部分切除 肝功能 

分 类 号：R285.5[医药卫生—中药学]