基于AMPK信号通路探讨花旗泽仁对2型糖尿病糖、脂代谢作用的实验研究  

Experimental Study on Glycolipid Metabolism of Type 2 DM with Huaqizeren Based on AMPK Signaling Pathway

作　　者：吴鑫宇[1] 黄云蕾 葛鹏玲[1] 张婷婷[1] 关子赫 WU Xinyu;HUANG Yunlei;GE Pengling;ZHANG Tingting;GUAN Zihe(Heilongjiang University of Chinese Medicine,Harbin 150040,China;93220 Troops of the Chinese People's Liberation Army,Harbin 150046,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]中国人民解放军93220部队,黑龙江哈尔滨150046

出　　处：《中医药学报》2021年第3期14-17,共4页Acta Chinese Medicine and Pharmacology

基　　金：黑龙江省自然科学基金(YQ2020H027);黑龙江省博士后特别资助(LDH-Z18246)。

摘　　要：目的:基于AMPK信号通路探讨花旗泽仁对2型糖尿病糖、脂代谢的作用机制。方法:db/m小鼠10只,为空白组;db/db小鼠40只,随机分为4组,每组10只,为模型组、阳性药组(0.2 g/kg)、花旗泽仁高剂量组(32 g/kg)、花旗泽仁低剂量组(16 g/kg)。给药6周后,检测各组小鼠空腹血糖(FPG)及血清中胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C),Western blot法检测各组小鼠肝脏中单磷酸腺苷酸活化蛋白激酶(AMPK)、促进葡萄糖转运体4(GLUT4)、瘦素(Leptin)、过氧化物酶体增殖活化受体-1a(PGC-1a)蛋白质相对表达量。结果:花旗泽仁可显著降低FPG及血清中TC、TG和HDL-C的含量,上调小鼠肝脏中AMPK、GLUT4、Leptin、PGC-1a蛋白质相对表达量。结论:花旗泽仁可显著改善db/db小鼠糖、脂代谢紊乱,其作用可能与调节肝脏中AMPK相关信号通路有关。Objective:To explore glycolipid metabolism effect of Huaqizeren on type 2 diabetes mellitus(Type 2 DM)based on AMPK signaling pathway.Methods:10 db/m mice were taken as the blank control;40 db/db mice were randomly divided into four groups,each with ten mice,which were the model group,the positive drug group(0.2 g/kg),Huaqizeren high-dose group(32 g/kg)and Huaqizeren low-dose group(16 g/kg).After 6 weeks of administration,the fasting blood glucose(FPG)and serum cholesterol(TC),triglyceride(TG)and low-density lipoprotein cholesterol(LDL-C)were detected in each group of mice;the Western blot method was used to detect the relative protein expressions of adenylate monophosphate activated protein kinase(AMPK),glucose transporter 4(GLUT4),Leptin and peroxisome proliferation activated receptor-1α(PGC-1α)in the livers of the mice.Results:Huaqizeren could significantly reduce the level of FPG and the serum contents of TC,TG and HDL-C,up-regulate the relative protein expressions of AMPK,GLUT4,Leptin and PGC-1α.Conclusion:Huaqizeren can significantly improve glucose and lipid metabolism disorders in db/db mice,and its effect may be related to the regulation of AMPK-related signaling pathway in the liver.

关 键 词：花旗泽仁 2型糖尿病 单磷酸腺苷酸活化蛋白激酶 葡萄糖转运体4 瘦素 过氧化物酶体增殖活化受体-1a 

分 类 号：R285.5[医药卫生—中药学]