飞行时间质谱法检测肉兔IGF-IR基因的多态性  

Rapid Detection of SNPs in Meat Rabbit IGF-IR Gene by Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

作　　者：梅秀丽[1,2] 邝良德 郭志强[1,2] 李娟 黄邓萍[1,2] 谢晓红 雷岷[1,2] 李丛艳[1,2] Mei Xiu-li;Kuang Liang-de;Guo Zhi-qiang;Li Juan;Huang Deng-ping;Xie Xiao-hong;Lei Min;Li Cong-yan(Sichuan Animal Science Academy,Chengdu 610066;Animal Breeding and Genetics key Laboratory of Sichuan Province,Chengdu 610066;Institute of Animal Science Chengdu Academy of Agriculture and Forestry Science Sichuan Province,Chengdu 611130)

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]动物遗传育种四川省重点实验室,成都610066 [3]四川省成都市农林科学院畜牧研究所,成都611130

出　　处：《中国养兔》2021年第1期4-7,15,共5页Chinese Journal of Rabbit Farming

基　　金：四川省科研院所基本科研业务费项目(2016YSKY0146、SASA201905);国家兔产业技术体系(CARS-43-D-3)。

摘　　要：应用MassARRAY单核苷酸多态性(single nucleotide polymorphism,SNP)基质辅助激光解吸电离飞行时间质谱(matrix assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF-MS)基因分型法检测肉兔胰岛素样生长因子1受体(Insulin-like Growth Factor 1 Receptor,IGF-IR)的多态性(SNPs),为今后建立肉兔辅助标记选择奠定基础。以家兔IGF-IR基因序列为模板,利用PCR扩增测序法筛查了肉兔IGF-IR基因的9个SNPs。为进一步验证筛选的准确性,应用MALDI-TOF-MS针对384只肉兔检测了5个SNPs。结果显示,平均检出率为98%,能够准确检测出3种常见的基因型。所检SNP位点的平均最小等位基因频率(MAF)为0.45,平均多态信息含量(PIC)为0.36。本研究运用的方法快速、可靠、准确度高,为以后开展肉兔生长性状基因的SNP分型和检测等奠定了基础。The study used MassARRAY single nucleotide polymorphism matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS)for determination of single nucleotide polymorphisms in meat rabbit IGF-IR gene,which provided a foundation for constructing the marker assisted selection strategy.The cDNA sequence of rabbit IGF-IR gene was screened rapidly based on PCR amplification and sequencing method,and in which 9 SNPs were found.To further identify the reliability of screening system,5 SNPs were genotyped in 384 meat rabbits using the MALDI-TOF-MS method.The results showed that 5 SNPs had an average of 98%in call rate,and in which three genotypes could be accurately distinguished.The average minor allele frequency(MAF)and polymorphism information content(PIC)value of the 5 markers was 0.45 and 0.36,respectively.Our findings indicated that the rapid determination method of MALDI-TOF-MS with PCR and sequencing with highly accurate properties was a useful tool for identifying the SNPs of rabbit IGF-IR gene that laid the foundations for studying SNP genotype in rabbit genes associated production traits.

关 键 词：肉兔 IGF-IR基因 飞行时间质谱 SNPS 

分 类 号：S829.1[农业科学—畜牧学]