BMSCs过表达BMP-4复合同种异体骨修复软骨缺损  

Repairment of Cartilage Defects through BMSCs Overexpression BMP-4 with Allogeneic Bone

作　　者：张飞 温福利 党源 薛来恩 郑和平 ZHANG Fei;WEN Fuli;DANG Yuan;XUE Laien;ZHENG Heping(The 900^th Hospital of the Joint Logistics Team Fuzhou,Fuzhou 350025,China;Shunde Hospital of Southern Medical University,Shunde 528300,China)

机构地区：[1]联勤保障部队第九○○医院基础医学实验室,福州350025 [2]南方医科大学顺德医院,顺德528300

出　　处：《实验动物科学》2021年第1期7-12,共6页Laboratory Animal Science

基　　金：军队实验动物专项科研课题(No.SYDW-2016-007)。

摘　　要：目的针对关节软骨损伤后修复困难,采用腺病毒(Ad)载体介导骨形态发生蛋白(BMP-4)修饰骨髓间充质干细胞(BMSCs),并复合同种异体骨软骨柱支架,以期实现关节软骨有效再生。方法制备同种异体骨软骨柱,BMSCs过表达BMP-4复合同种异体骨修复关节软骨损伤,膝关节标本取材大体观察及分析,HE、番红固绿、Masson及免疫组化检软骨再生情况。结果 HE、番红固绿、Masson及免疫组化均提示实验组能显著促进缺损再生,并提高新生组织软骨含量。结论过表达BMP-4复合同种异体骨支架能显著促进软骨缺损再生,修复软骨损伤。Objective In our research,we used BMSCs modified by BMP-4 through adenovirus and combined with allogeneic bone scaffold in order to have an effective regeneration of articular cartilage. Method Firstly,manufacturing the allogeneic bone cartilage column grafts,then repairing the articular cartilage defects with allogeneic bone cartilage column grafts which are modified by BMSCs overexpressing BMP-4. After that,taking a general observation and analysis of knee joint specimens. Lastly,taking a quantitative analysis of the regeneration of articular cartilage through the HE,saffron O,Masson and immunohistochemistry. Result The result of the HE,safranine O,Masson and immunohistochemistry indicated that the experimental group could significantly increase the content of cartilage fibers in the regeneration of articular cartilage. Conclusion The composite of allogeneic bone scaffolds with BMSCs overexpressing BMP-4 can significantly promote the regeneration of cartilage cartilage and the repairment of cartilage damage.

关 键 词：骨髓间充质干细胞(BMSCs) 骨形态发生蛋白(BMP-4) 同种异体骨 

分 类 号：R684[医药卫生—骨科学]