运动对高脂饲养大鼠白色脂肪组织血管内皮生成相关因子的影响  

Effects of exercise on vascular endothelial growth related factors in white adipose tissue of high-fat fed rats

作　　者：朱文阁 宋鸽 邓懿敏 陈君颖 严翊[1] ZHU Wenge;SONG Ge;DENG Yimin(Beijing Sport University,100084)

机构地区：[1]北京体育大学运动人体科学学院,北京市100084

出　　处：《中国康复医学杂志》2021年第4期398-405,共8页Chinese Journal of Rehabilitation Medicine

基　　金：中央高校基本科研业务费专项资金资助项目(2018PT015,2019PT011);国家自然科学基金资助项目(32071173)。

摘　　要：目的:观察8周中等强度有氧运动干预以及高脂饮食对雄性大鼠血管内皮生成相关因子的影响。方法:40只3周龄SPF级雄性SD大鼠适应性喂养3天后,随机分为标准安静组(CS)、标准运动组(CE)、高脂安静组(HS)、高脂运动组(HE)。标准组大鼠喂饲普通饲料(D12450B),高脂组大鼠喂饲高脂饲料(D12451),所有大鼠均自由饮食、饮水,CE、HE组大鼠进行8周运动干预(60%—70%VO2max,1h/次,5次/周,每次训练内容包括5min热身,50min跑台训练,5min恢复)。均在8周干预后取材(皮下脂肪组织、睾周脂肪组织和肾周脂肪组织)。采用荧光定量PCR(RT-qPCR)法测血管内皮生成相关因子VEGF、VEGFR2、CD31的mRNA相对表达量。Western Blot检测皮下及睾周白色脂肪组织中解偶联蛋白-1(UCP-1)蛋白表达水平。结果:①与CS组相比,HS组大鼠皮下脂肪组织中VEGFR2、CD31 mRNA的相对表达量降低(P<0.05),UCP-1蛋白表达量显著降低(P<0.01),睾周脂肪组织中CD31 mRNA的相对表达量显著性升高(P<0.01),UCP-1蛋白表达水平显著降低(P<0.01),肾周脂肪组织中VEGF、VEGFR2、CD31 mRNA的相对表达量显著性升高(P<0.01);②与HS组相比,HE组大鼠皮下脂肪组织中VEGFR2 mRNA的相对表达量显著性升高(P<0.01),UCP-1蛋白表达量显著性降低(P<0.01),睾周脂肪组织中VEGF、VEGFR2、CD31 mRNA的相对表达量均显著性降低(P<0.01),UCP-1蛋白表达水平也显著降低(P<0.01),肾周脂肪组织中VEGF、VEGFR2、CD31 mRNA的相对表达量均显著性升高。结论:8周高脂饮食及运动干预对大鼠白色脂肪组织血管生成相关因子mRNA表达量的影响具有组织差异性,其作用机制有待于进一步探究。Objective:To observe the effects of 8-week moderate-intensity aerobic exercise and high-fat diet on vascular endothelial growth-related factors of male rats.Method:After adjustable fed for 3 days,forty three-week-old SPF male SD rats were randomly divided into standard quiet group(CS),standard exercise group(CE),high-fat quiet group(HS),and high-fat exercise group(HE).Rats in the standard group were fed with normal diet feed(D12450B),while high-fat diet feed(D12451)in the high-fat group.All rats were free to eat and drink,and rats in the CE and HE groups underwent exercise intervention for 8 weeks(60%—70%VO2max,1h/time,5 times/week,each training includes 5min warm-up,50min treadmill training,5min recovery).All materials were taken after 8 weeks of intervention(inguinal adipose tissue,epididymal white adipose tissue and perirenal white adipose tissue).The relative expression levels of VEGF,VEGFR2,and CD31 mRNA were measured by RT-qPCR.Western Blot was used to detect the expression of uncoupling protein 1(UCP-1)in inguinal adipose tissue and epididymal white adipose tissue.Result:①Compared with the CS group,the relative expressions of VEGFR2 and CD31 mRNA in the inguinal adipose tissue of the HS group were significantly reduced(P<0.05),and the UCP-1 protein expression was also significantly reduced(P<0.01).The relative expression of CD31 mRNA in epididymal white adipose tissue were significantly increased(P<0.01),the expression level of UCP-1 protein was significantly reduced(P<0.01).The relative expressions of VEGF,VEGFR2,CD31 mRNA in perirenal white adipose tissue were significantly increased(P<0.01).②Compared with the HS group,the relative expression of VEGFR2 mRNA in the inguinal adipose tissue of the HE group was significantly increased(P<0.01),the protein expression of UCP-1 was significantly reduced(P<0.01).The relative expressions of VEGF,VEGFR2,and CD31 mRNA in tepididymal white adipose tissue were significantly reduced(P<0.01),and UCP-1 protein expression was also significantly reduced(P<0.01).The r

关 键 词：运动干预 高脂饲养 白色脂肪组织 棕色脂肪组织 血管内皮生成相关因子 白色脂肪组织棕色化 

分 类 号：R493[医药卫生—康复医学]