烟草NtbZIP038的克隆、鉴定及表达模式分析  

Cloning,Identification and Expression Pattern Analysis of Tobacco NtbZIP038

作　　者：赵泽玉 李志远 孙晋浩 李东徽 解敏敏[1] 陈明丽[1] 龚达平[1] ZHAO Zeyu;LI Zhiyuan;SUN Jinhao;LI Donghui;XIE Minmin;CHEN Mingli;GONG Daping(Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院烟草研究所,青岛266101 [2]中国农业科学院研究生院,北京100081

出　　处：《中国烟草科学》2021年第2期71-76,83,共7页Chinese Tobacco Science

基　　金：中国烟草总公司烟草基因组计划重大专项项目(110201801024);湖南中烟工业有限责任公司科技项目(202043000834065)。

摘　　要：碱性亮氨酸拉链(bZIP)转录因子是植物中数量最多、最具有多样性的基因家族之一,对植物生长发育及胁迫响应具有重要作用。为探究烟草中bZIP转录因子的生物学功能,本研究在烟草K326中克隆得到一个bZIP转录因子基因NtbZIP038,并对其保守结构域进行分析,利用qRT-PCR技术检测了该基因的表达模式,进一步结合亚细胞定位及转录激活试验对其功能进行了初步鉴定。结果表明,NtbZIP038含有典型的bZIP结构域,亚细胞定位于细胞核中并具有转录激活活性。表达模式分析表明,NtbZIP038在根中表达量最高(p<0.001),并在盐、干旱、冷、热、脱落酸(ABA)等非生物胁迫下都有明显的表达量变化,在ABA处理下尤其明显(p<0.001)。因此,作为bZIP家族中的一员,NtbZIP038转录因子可能在烟草根系发育、ABA响应与非生物胁迫中扮演着重要的角色。The basic leucine zipper(bZIP)transcription factor comprise one of the largest and most diverse gene families in plants,which are important in plant growth and response to stresses.In order to explore the biological functions of the bZIP transcription factors in tobacco,a bZIP gene family transcription factor NtbZIP038 in K326 was cloned and its conserved domains were analyzed.Furthermore,the gene expression pattern was studied using qRT-PCR technology and subcellular localization analysis.Finally,transcription activation experiments were performed to identify its potential function as a transcription factor.The results showed that NtbZIP038 contains a typical bZIP domain.The NtbZIP038 protein was localized in the nucleus and had transactivation activity.The expression pattern analysis showed that NtbZIP038 were expressed with the highest level in the root(p<0.001),and there were obvious expression changes under abiotic stresses such as salt,drought,cold,heat,ABA,etc.,especially after ABA treatment(p<0.001).Therefore,NtbZIP038,a typical bZIP transcription factor,may play an important role in root development,ABA response and abiotic stress response in tobacco.

关 键 词：BZIP 烟草 转录因子 ABA 非生物胁迫 

分 类 号：S572[农业科学—烟草工业]