利多卡因抑制前列腺癌PC-3细胞恶性生物学行为和ERK/JNK的磷酸化  

Lidocaine Inhibits Malignant Biological Behaviors and ERK/JNK Phosphorylation in Prostate Cancer PC-3 Cells

作　　者：江毅峰[1] 陈丹[1] 杨雄 黄健[1] JIANG Yifeng;CHEN Dan;YANG Xiong;HUANG Jian(Department of Urology,Chengdu Seventh People’s Hospital,Chengdu,610041,China)

机构地区：[1]成都市第七人民医院泌尿外科,成都市610041

出　　处：《医学分子生物学杂志》2021年第2期134-139,共6页Journal of Medical Molecular Biology

基　　金：四川省科技攻关项目(No.2018JY0229)。

摘　　要：目的 研究利多卡因对前列腺癌PC-3细胞恶性生物学行为和细胞外调节蛋白激酶/c-Jun氨基末端激酶(ERK/JNK)磷酸化的影响.方法 以PC-3细胞为模型进行研究.分组:对照组、利多卡因处理组(低25μmol/L、中50μmol/L、高100μmol/L).采用克隆实验检测细胞增殖;流式检测凋亡和线粒体膜电位;Transwell实验检测细胞侵袭能力;Western印迹检测血管内皮生长因子(vascular endothelial growth factor,VEGF),E-cadherin和Vimentin的蛋白表达和ERK1/2和JNK1/2的磷酸化情况.结果 与对照组比较,利多卡因处理可抑制PC-3细胞增殖、侵袭和迁移,促进其线粒体膜电位的变化和凋亡.Western印迹结果显示,利多卡因可抑制PC-3细胞中ERK1/2和JNK1/2的磷酸化水平.结论 利多卡因处理可有效地抑制前列腺癌PC-3细胞恶性生物学行为,其潜在机制或与利多卡因对ERK/JNK的磷酸化的抑制有关.Objective To study the effect of lidocaine on the malignant biological behaviors of prostate cancer PC-3 cells and the phosphorylation of extracellular regulatory protein kinase/c-Jun Nterminal kinase(ERK/JNK).Methods Prostate cancer PC-3 cells were used as a model.Grouping was as follows:a control group,a lidocaine treatment group,which was further divided into a low concentration(25μmol/L),a medium concentration(50μmol/L)and a high concentration(100μmol/L)group.Clonal formation was used to detect cell proliferation.Flow cytometry was used to detect the change of apoptosis and mitochondrial membrane potential.Transwell test was used to detect cell invasion.Western blotting was used to detect the protein expression of vascu-lar endothelial growth factor(VEGF),E-cadherin and vimentin,and phosphorylation of ERK1/2 and JNK1/2.Results Compared with the control group,lidocaine significantly inhibited the proliferation,invasion and migration of PC-3 cells,and promoted the change of mitochondrial membrane potential and apoptosis.Western blot results showed that lidocaine inhibited the phosphorylation of ERK1/2 and JNK1/2 in PC-3 cells.Conclusion Lidocaine can effectively inhibit the malignant biological behaviors of PC-3 cells,which may be related to the inhibition of ERK/JNK phosphorylation by lidocaine.

关 键 词：利多卡因 前列腺癌 线粒体膜电位 EMT VEGF 

分 类 号：R332[医药卫生—人体生理学]