2016—2019年温岭市禽流感外环境监测结果分析  

Analysis of surveillance results for avian influenza in external environment in Wenling City from 2016 to 2019

作　　者：余建 陈宇鸿[1] YU Jian;CHEN Yu-hong(Wenling Center for Disease Control and Prevention,Wenling,Zhejiang 317500,China)

机构地区：[1]温岭市疾病预防控制中心,浙江温岭317500

出　　处：《中国公共卫生管理》2021年第2期211-213,共3页Chinese Journal of Public Health Management

摘　　要：目的了解2016—2019年温岭市外环境中禽流感病毒亚型及分布情况,为禽流感的防控提供参考依据。方法采集2016—2019年温岭市活禽市场和禽类养殖场中各类标本,采用实时荧光定量PCR检测A型、H5、H7、H9和N9亚型流感病毒核酸,并建立数据库进行分析。结果2016—2019年共采集样本621份,不同年份间样品阳性率分别为72.97%、54.17%、59.49%和30.68%,差异有统计学意义(χ^(2)=34.19,P<0.05);城区样本阳性率为60.37%,高于农村样本的50.17%,差异有统计学意义(χ^(2)=6.51,P<0.05);流行季节(10月至次年3月)不同月份监测数据显示阳性率分别为53.33%、44.64%、72.46%、65.85%、46.59%和36.62%,差异有统计学意义(χ^(2)=32.79,P<0.05)。在所有阳性亚型中,以H9亚型为主,阳性率为54.49%。结论温岭市外环境禽流感病毒感染以H9和H7及二者相关的混合感染为主,存在基因重配的可能性,需对活禽市场和养殖场进行持续监测,尤其是阳性率高发的12月和1月更需加强防控,做好宣传教育工作。Objective To understand the subtype and distribution of avian influenza virus in the external environment of Wenling City from 2016 to 2019,so as to provide reference for the prevention and control of avian influenza.Methods All kinds of samples were collected from the live poultry market and poultry farm in Wenling City from 2016 to 2019.The nucleic acids of influenza A,H5,H7,H9 and N9 subtypes were detected by real-time fluorescent quantitative PCR,and the database was established for analysis.Results From 2016 to 2019,621 samples were collected.The positive rates of the samples in different years were 72.97%,54.17%,59.49%and 30.68%respectively,which had statistical significance(χ^(2)=34.19,P<0.05).The positive rate of urban samples was 60.37%,which was higher than that of rural samples by 50.17%.The difference is statistically significant(χ^(2)=6.51,P<0.05).The monitoring data of different months in the epidemic season(October to March of the next year)showed that the positive rates were 53.33%,44.64%,72.46%,65.85%,46.59%and 36.62%respectively,with statistically significant difference(χ^(2)=32.79,P<0.05).Conclusion H9 and H7 and their related mixed infection are the main infectious factors of avian influenza virus in the outer environment of Wenling City.There is a possibility of gene recombination.It is necessary to continuously monitor the live poultry market and farms,especially in December and January when there is a high incidence of positive virus.It is also necessary to strengthen prevention and control,and do a good job in publicity and education.

关 键 词：禽流感 外环境 核酸检测 流行季节 

分 类 号：R858[医药卫生—航空、航天与航海医学]