β-榄香烯抗DDP耐药卵巢癌细胞SKOV3/DDP作用及机制研究  

Effect and Mechanism ofβ-Elemene on DDP Resistant SKOV3/DDP Ovarian Cancer

作　　者：陈心蕊 周宋汇[1] 汪瑞辰 许丽 CHEN Xinrui;ZHOU Songhui;WANG Ruicheng;XU Li(Jiangsu Cancer Hospital,Jiangsu Institute of Cancer Research,the Affiliated Cancer Hospital of Nanjing Medical University,Nanjing,Jiangsu 210009,China)

机构地区：[1]江苏省肿瘤医院,江苏省肿瘤防治研究所,南京医科大学附属肿瘤医院,南京210009

出　　处：《药学与临床研究》2021年第2期81-85,共5页Pharmaceutical and Clinical Research

摘　　要：目的:研究β-榄香烯对DDP耐药卵巢癌细胞SKOV3/DDP增殖生长、逆转耐药的作用,以及对耐药细胞中耐药相关蛋白表达的影响。方法:CCK-8法研究β-榄香烯对SKOV3/DDP细胞增殖的抑制作用;克隆形成实验研究β-榄香烯对SKOV3/DDP细胞克隆形成的影响;流式细胞术研究β-榄香烯对SKOV3/DDP细胞凋亡的影响;CCK-8法研究β-榄香烯对SKOV3/DDP细胞产生顺铂(DDP)耐药的逆转指数;Western Blot法研究β-榄香烯对SKOV3/DDP细胞中耐药相关蛋白ABCB1、LRP和P-gp表达水平的影响。结果:β-榄香烯在20、40、80μmol·L^(-1)时能够抑制SKOV3/DDP细胞的增殖、克隆形成并促进细胞凋亡;β-榄香烯在不具有直接杀伤细胞作用的10μmol·L^(-1)时即可以逆转SKOV3/DDP细胞对DDP的耐药,其逆转耐药指数为2.58倍;β-榄香烯在20、40、80μmol·L^(-1)时能够减少耐药相关蛋白ABCB1、LRP和P-gp表达。结论:β-榄香烯能够直接抑制SKOV3/DDP细胞增殖、促进其凋亡并逆转细胞DDP耐药,其作用机制可能与减少耐药相关蛋白ABCB1、LRP和P-gp表达有关。Objective:To investigate the effect ofβ-elemene on the proliferation and reversal of drug resistance of SKOV3/DDP ovarian cancer cells,and its effect on the expression of resistance associate proteins.Methods:CCK-8 method was used to test the inhibitory effect ofβ-elemene on the proliferation of SKOV3/DDP cells;Clone formation experiment was used to study the effect ofβ-lemene on the colony formation of SKOV3/DDP cells;Flow cytometry was used to study the effect ofβ-lemene on the apoptosis of SKOV3/DDP cells;CCK-8 method was used to calculate the reversal index of DDP resistance of SKOV3/DDP cells;Western blot was used to determine the protein expression of ABCB1,LRP and P-gp in SKOV3/DDP cells.Results:β-Elemene inhibited the proliferation and clone formation while promoted the apoptosis of SKOV3/DDP cells at 20,40 and 80μmol·L^(-1);β-Elemene reversed the resistance of SKOV3/DDP cells to DDP at 10μmol·L^(-1) without direct killing effects,and the reversal index was 2.58;β-Elemene reduced the expression of ABCB1,LRP and P-gp at 20,40 and 80μmol·L^(-1).Conclusion:β-Elemene can directly inhibit the proliferation,promote the apoptosis and reverse the DDP resistance of SKOV3/DDP cells,and the mechanism may be related to the decrease of ABCB1,LRP and P-gp expression.

关 键 词：卵巢癌 耐药 Β-榄香烯 顺铂 

分 类 号：R965.1[医药卫生—药理学]