离子色谱法测定口服电解质颗粒中阴、阳离子的含量  

Determination of Anions and Cations in Oral Electrolyte Granules by Ion Chromatography

作　　者：张建茹 昝艳楠 丁逸梅[1,2] ZHANG Jianru;ZAN Yannan;DING Yimei(School of Pharmacy,Nanjing Tech University,Nanjing 211800,China;Jiangsu Institute of Materia Medica,Nanjing 211800,China)

机构地区：[1]南京工业大学药学院,南京211800 [2]江苏省药物研究所,南京211800

出　　处：《药学与临床研究》2021年第2期101-105,共5页Pharmaceutical and Clinical Research

摘　　要：目的:建立离子色谱法(IC)测定口服电解质颗粒中6种阴、阳离子(氯离子、磷酸根、柠檬酸根离子、钠离子、钾离子、镁离子)含量的方法。方法:测定阴离子的色谱柱为Metrosep A Supp-5 column(250 mm×4 mm),流动相为12.8 mmol·L^(-1)Na2CO3,等度洗脱,柱温为30℃,流速为0.7 mL·min^(-1),进样量为20μL,电导检测器;测定阳离子的色谱柱为Metrosep C4column(250 mm×4 mm),流动相为5 mmol·L^(-1)HNO_(3),等度洗脱,柱温为30℃,流速为0.7 mL·min^(-1),进样量为20μL,电导检测器。结果:在各色谱条件下,电解质颗粒中各阴阳离子之间分离良好;专属性良好;线性关系良好(r=0.9999);精密度及平均加样回收率(n=9)RSD均<2.0%,且回收率在98%~103%。结论:该方法操作简便、灵敏度高、选择性好,适用于常规样品分析。Objective:This article is to establish an ion chromatography method for determining the contents of three anions(sodium,potassium,magnesium)and three cations(chloride,phosphate,citrate)in oral granules of electrolytes.Methods:The anion chromatographic column was Metrosep A SUPP 5-150 column(250 mm×4 mm)equipped with an Dionex IonPac AG23 guard column(50 mm×4 mm)and eluted with isocratic 12.8 mmol·L^(-1)Na2CO3;the canion chromatographic column was Metrosep C4 column(250 mm×4 mm)equipped with an Metrosep C4 guard column(250 mm×4 mm)and eluted with isocratic 5 mmol·L^(-1)HNO_(3.)The flow rates were both 0.7 mL·min-1.The injection volume was 20μL and the column temperature was 30℃.Results:Good separation for the anions(chloride,phosphate,citrate)and cations(sodium,potassium,magnesium)were achieved with good specificity and linear relationship(r=0.9999),the RSD of injection precision,repeatability and sample recovery rate were within 2.0%,and the sample recovery rates were between 98%and 103%.Conclusions:The method is simple in operation,high in sensitivity and good in selectivity.

关 键 词：离子色谱法 电解质颗粒 阴离子 阳离子 

分 类 号：R927.2[医药卫生—药学]