永州丹参中丹参酮ⅡA含量测定及提取工艺优化  

Determination and Optimization of TanshinoneⅡA Content of Salvia Miltiorrhiza in Yongzhou

作　　者：马欣然 苏伟康 靳琦 何勇辉 陈小明 MA Xin-ran;SU Wei-kang;JIN Qi;HE Yong-hui;CHEN Xiao-ming(School of Chemistry and Bioengineering,Hunan University of Science and Engineering,Hunan Yongzhou 425100;Hunan Provincial Engineering Research Center for Ginkgo Folium,Hunan University of Science and Engineering,Hunan Yongzhou 425100;Hunan HENGWEI Pharmaceutical Co.,Ltd.,Hunan Yongzhou 425100,China)

机构地区：[1]湖南科技学院化学与生物工程学院,湖南永州425100 [2]湖南科技学院湖南省银杏工程技术研究中心,湖南永州425100 [3]湖南恒伟药业股份有限公司,湖南永州425100

出　　处：《广州化工》2021年第8期89-92,共4页GuangZhou Chemical Industry

基　　金：科技特派员创新创业项目(No.2019NK4153)。

摘　　要：用乙醇回流提取法以丹参中丹参酮ⅡA含量为指标优化提取工艺。在单因素实验的基础上,对温度、料液比、浸泡时间、回流时间4个因素按照L_(9)(3^(4))正交表进行正交试验。再将所得供试品用高效液相色谱进行测定。研究发现,丹参中丹参酮ⅡA的最佳提取条件为温度70℃、料液比1:8、浸泡时间0.5 h、回流时间2 h,且根据峰面积得出丹参酮ⅡA含量2.010 mg/g。结果表明,高效液相色谱是测定丹参中丹参酮ⅡA含量科学、简洁、准确、有效的检测方法。The optimum extraction process of tanshinoneⅡA in Salvia miltiorrhiza by ethanol reflux extraction was studied.On the basis of single factor experiment,the orthogonal test was carried out according to the orthogonal table of four factors:temperature,ratio of material to liquid,soaking time and reflux time,according to the orthogonal table of four factors and three levels.The desired sample was determined by high performance liquid chromatography(HPLC).The results showed that the optimum extraction conditions of tanshinoneⅡA in Salvia miltiorrhiza were as follows:temperature was 70℃,material-liquid ratio was 1:8,soaking time was 1 h,reflux time was 2 h.According to the peak area,the content of tanshinoneⅡA was obtained 2.010 mg/g.This experiment showed that high performance liquid chromatography was a scientific,simple,accurate and effective method for the determination of tanshinone in Salvia miltiorrhiza.

关 键 词：丹参 丹参酮ⅡA 乙醇回流提取法 正交实验法 HPLC 

分 类 号：R284.2[医药卫生—中药学]