钩藤不同部位生物碱类成分的含量测定及其质量评价  

Determination and Quality Evaluation of Alkaloids in Different Parts of Uncaria

作　　者：刘小芬 蔡洪鲲 王琼 胡嘉璇 聂海澜 蒋范任 LIU Xiao-fen;CAI Hong-kun;WANG Qiong;HU Jia-xuan;NIE Hai-lan;JIANG Fan-ren(Yiyang Medical College,Hunan Yiyang 413000;Yiyang Institute for Food and Drug Control,Hunan Yiyang 413000,China)

机构地区：[1]益阳医学高等专科学校,湖南益阳413000 [2]益阳市食品药品检验所,湖南益阳413000

出　　处：《广州化工》2021年第8期153-155,共3页GuangZhou Chemical Industry

基　　金：益阳市科技局项目(No.2017YZ18);湖南省教育厅项目(No.18C1729)。

摘　　要：采用反相高效液相色谱法,对钩藤不同部位异钩藤碱、钩藤碱、去氢钩藤碱、异去氢钩藤碱4种生物碱类成分进行含量测定。采用Gemini NX C18(250 mm×4.6 mm,5μm)色谱柱,以1%醋酸铵(A)-乙腈(B)为流动相进行梯度洗脱,流速1.0 mL·min^(-1);检测波长为245 nm;柱温25℃;进样量10μL。本方法简便可靠,专属性强,重现性好,适用于钩藤中生物碱的含量测定;钩藤不同部位生物碱类成分含量差异显著。The contents of isorhynchophylline;rhynchophylline,corynoxeine and isocorynoxeine in different parts of Uncaria were determined by RP-HPLC.The method was performed on a Gemini NX C_(18) chromatographic column(250 mm×4.6 mm,5μm),with 1%ammonium acetate(A)-acetonitrile(B)as the mobile phase with gradient elution,the flow rate was 1.0 mL·min^(-1),the detect wavelength was 245 nm,the column temperature was 25℃,and the injection volume was 10μL.The method was simple,reliable,specific and reproducible.It was suitable for the determination of alkaloids in Uncaria.The contents of alkaloids in different parts of Uncariawere significantly different.

关 键 词：钩藤 异钩藤碱 钩藤碱 去氢钩藤碱 异去氢钩藤碱 反相高效液相色谱法 不同部位 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]