小儿肝炎颗粒多成分含量测定  

Determination of Multiple Components in Xiao′er Ganyan Granules

作　　者：熊晓毅[1] 王剑[2] XIONG Xiao-yi;WANG Jian(Xianyang Vocational and Technical College,Xianyang 712000,China;Weinan Vocational and Technical College,Weinan 714026,China)

机构地区：[1]咸阳职业技术学院,陕西咸阳712000 [2]渭南职业技术学院,陕西渭南714026

出　　处：《中国现代中药》2021年第3期542-547,共6页Modern Chinese Medicine

摘　　要：目的:建立小儿肝炎颗粒中7种特征成分的高效液相色谱法(HPLC)含量测定方法。方法:采用Agilent-ODS色谱柱(250 mm×4.6 mm,5μm),以乙腈(A)-0.1%磷酸溶液(B)为流动相进行梯度洗脱,流速为1.0 mL·min-1,绿原酸、滨蒿内酯检测波长为330 nm,栀子苷、大豆苷、盐酸小檗碱检测波长为250 nm,盐酸黄柏碱、黄芩苷检测波长为280 nm,柱温为30℃,进样量为10μL。结果:绿原酸、滨蒿内酯、大豆苷、栀子苷、盐酸小檗碱、盐酸黄柏碱、黄芩苷进样量分别在4.342~86.848、1.361~27.216、2.082~41.644、4.666~93.328、6.025~120.504、1.971~39.420、8.243~164.856μg·mL^(-1)与峰面积呈良好线性关系(r为0.9995~0.9999);平均加样回收率分别为101.2%、99.2%、99.5%、99.8%、99.5%、99.6%、99.6%(RSD<1.5%,n=6);精密度、重复性、稳定性(48 h)试验的RSD均小于2.0%(n=6)。6批小儿肝炎颗粒中7种成分均有检出,但含量存在差异。结论:建立的HPLC准确可靠、专属性好,可用于小儿肝炎颗粒中绿原酸等7种活性成分含量的同时测定。Objective:To establish an HPLC method for the simultaneous content determination of seven pharmaceutical companies in Xiao′er Ganyan granules.Methods:The determination was performed on Agilent-ODS column(250 mm×4.6 mm,5μm)with mobile phase consisted of acetonitrile-0.1%phosphoric acid(gradient elution)at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 330 nm for chlorogenic acid and scoparone,250 nm for gardenin、daidzin and berberine hydrochloride,280 nm for cyperine hydrochloride and astragalin.The column temperature was set at 30℃and the injection volume was 10μL.Results:Good linear correlations(r=0.9995-0.9999)were found in the range of 4.342-86.848,1.361-27.216,2.082-41.644、4.666-93.328,6.025-120.504,1.971-39.420,8.243-164.856μg·mL^(-1)for chlorogenic acid,scoparone,daidzin,gardenin,berberine hydrochloride,cyperine hydrochloride,astragalin.Average recoveries were 101.2%,99.2%,99.5%,99.8%,99.5%,99.6%,99.6%,respectively(RSD<1.5%,n=6).All the seven components were detected in the six samples but their content varied.RSDs of precision,reproducibility and stability tests(48 h)were lower than 2.0%(n=6).Conclusion:The established HPLC method is accurate,reliable and specific,and suitable for simultaneous determination of seven active components as chlorogenic acid in Xiao′er Ganyan granules.

关 键 词：小儿肝炎颗粒 高效液相色谱法 含量测定 

分 类 号：R286[医药卫生—中药学]