分子伴侣蛋白介导的革兰阴性菌耐酸机制研究进展  

Advances in research progress on acid tolerance mechanism of Gramnegative bacteria mediated by molecular chaperone protein

作　　者：朱浩 刘楠[1] ZHU Hao;LIU Nan(School of Life Science and Technology,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]中国药科大学生命科学与技术学院,南京210009

出　　处：《中国药科大学学报》2021年第2期164-170,共7页Journal of China Pharmaceutical University

基　　金：国家重点研发计划资助项目(No.2018YFA0902000)。

摘　　要：微生物在酸胁迫下的耐受能力对于菌株的生长和工业化生产均具有重要意义。当细菌细胞面临外界酸性环境压力时,周质空间蛋白质相对胞内蛋白受到更大酸性压力,所受酸性伤害也比胞内蛋白质更为严重。在革兰阴性菌耐酸过程中,除了胞内的脱羧酶系统外,分子伴侣作为一种重要的"纠错"机制可参与识别并保护蛋白质的空间结构。本文介绍了HdeA、HdeB、DnaK和GroEL等分子伴侣在功能、结构、耐酸机制以及应用前景等方面的研究现状,阐述了分子伴侣介导的耐酸机制的调控方式。深入探究和解析分子伴侣对酸胁迫环境的生理适应策略,以期利用这些研究对目的菌株进行生理性能改造,提高菌株在酸性胁迫环境下的存活能力和耐受性,对工业化发酵、临床抗菌治疗靶点的发现等具有重要意义。The tolerance of microorganism under acid stress is of significant importance to the growth and indus⁃trial production of bacterial strain.When the bacterial cells are exposed to the external acid environment,proteins in periplasmic space are under higher acid stress and are thusmore susceptible to severe damage from acid than the intracellular protein,and these are also more susceptible to severe damage from acid than the intracellular protein.During the acid-resisting process of Gram-negative bacteria,in addition to the intracellular decarboxylase system,the molecular chaperone can also participate in the identification and protection of the space structure of protein as an important"correcting"mechanism.This paper,reviews the current researches on the function,structure,acid-resisting mechanism of variousmolecular chaperones,including HdeA,HdeB,DnaK and GroEL.Finally,the research progress of the acid-resisting mechanism among Gram-negative bacteria is summarized.Through in-depth investigation and analysis of the physiological adaptation strategy of molecular chaperone to the acid stress environment,this study can help to conduct physiological property modification of target strain and improve their viability and tolerance in acid stress environment,which has its important theoretical and prac⁃tical significance.

关 键 词：革兰阴性菌 大肠埃希菌 酸胁迫 周质空间 分子伴侣 进展 

分 类 号：R378[医药卫生—病原生物学]