Protective effect and mechanisms of action of Mongolian medicine Sulongga-4 on pyloric ligation-induced gastroduodenal ulcer in rats  

作　　者：Shan Tong Huan Wang Li-Sha A Ta-Na Bai Ju-Hua Gong Wen-Jie Jin Li-Li Dai Gen-Na Ba Sung-Bo Cho Ming-Hai Fu 

机构地区：[1]School of Mongolian Medicine,Inner Mongolia University for Nationalities,Tongliao 028000,Inner Mongolia Autonomous Region,China [2]Mongolian Medicine Surgery Department,Affiliated Hospital of Inner Mongolia University for Nationalities,Tongliao 028000,Inner Mongolia Autonomous Region,China [3]Traditional Mongolian Medicine Research Institute,Inner Mongolia University for Nationalities,Tongliao 028000,Inner Mongolia Autonomous Region,China

出　　处：《World Journal of Gastroenterology》2021年第16期1770-1784,共15页世界胃肠病学杂志（英文版）

基　　金：Mongolian Medicine Food and Drug Source Protection and Utilization Innovation Team Construction Project,No.190301;National Natural Science Foundation of China,No.81760765;Inner Mongolia University for Nationalities Doctoral Start-up Grant,No.BS412 and No.BS413;Mongolian Medicine Engineering Technology Research Centre Open Fund Project,No.MDK2017072;Inner Mongolia Autonomous Region Talent Development Fund Project,No.RC201802.

摘　　要：BACKGROUND Sulongga-4(SL-4)is a herbal formula used in traditional Mongolian medical clinics for the treatment of peptic ulcers and gastroenteritis,even though its pharmacological mechanism has not been well characterized.AIM To evaluate the protective effect and identify the mechanisms of action of SL-4 on gastroduodenal ulcer induced by pyloric ligation(PL)in rats.METHODS PL was performed to induce gastric and duodenal ulcers in rats,which were then treated with oral SL-4(1.3,2.6,or 3.9 g/kg per day)for 15 d.PL-induced gastroduodenal ulceration.Therapeutic effects were characterized by pathological and histological evaluations and inflammatory indicators were analyzed by enzyme-linked immunosorbent assay.Microarray analyses were conducted to identify gene expression profiles of gastroduodenal tissue in PL rats with or without SL-4 treatment.The candidate target genes were selected and verified by quantitative reverse transcription polymerase chain reaction(qRT-PCR).RESULTS SL-4 decreased histopathological features in the PL-induced ulcerated rats.SL-4 significantly (P < 0.05) decreased expression of tumor necrosis factor-α,interleukin (IL)-1β, IL-6, endotoxin, platelet-activating factor, and increasedprostaglandin E2 and epidermal growth factor in ulcer tissue. Microarray analysiswas used to identify a panel of candidate target genes for SL-4 acting on PLinducedulceration. Genes included some complement and coagulation cascadeand retinol metabolism pathways that are closely associated with inflammatoryresponses and gastric mucosal protective mechanisms. qRT-PCR showed thataltered expression of the selected genes, such as CYP2b2, UGT2b1, A2m, andMASP1 was consistent with the microarray results.CONCLUSIONSL-4 exerts protective effects against PL-induced gastroduodenal ulcers viareducing inflammatory cytokines and elevating expression of gastric acidinhibitory factors. Downregulation of CYP2b2 and UGT2b1 genes in retinolmetabolism and upregulation of A2m and MASP1 genes in the complement andcoagulation cascades

关 键 词：Sulongga-4 Peptic ulcer Pyloric ligation Microarray analysis Inflammatory reaction Retinol metabolism 

分 类 号：R29[医药卫生—民族医学]