赤松混交林枯梢病不同病级针叶内部细菌多样性研究  

Endosphere Bacterial Diversity within Mixed Forest of Pinus densiflora Infected by Different Incidences of Sphaeropsis sapinea

作　　者：谢宪 梁军[1,2] 朱彦鹏 时良[2] 高燕 张铭 程元 张星耀 XIE Xian;LIANG Jun;ZHU Yan-peng;SHI Liang;GAO Yan;ZHANG Ming;CHENG Yuan;ZHANG Xing-yao(Key Laboratory of Forest Protection of National Forestry and Grassland Administration,Research Institute of Forest Ecology,Environment and Protection,Chinese Academy of Forestry,Beijing 100091,China;Kunyushan Forest Ecosystem Research Station,Yantai 264100,Shandong,China;Chinese Research Academy of Environmental Sciences,Beijing 100012,China)

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业和草原局森林保护学重点实验室,北京100091 [2]昆嵛山森林生态系统国家定位观测研究站,山东烟台264100 [3]中国环境科学研究院,北京100012

出　　处：《林业科学研究》2021年第2期122-130,共9页Forest Research

基　　金：国家重点研发计划课题(2018YFC1200400);国家自然科学基金面上项目(31270682);山东昆嵛山森林生态系统国家定位观测研究站运行补助(2019132127)。

摘　　要：[目的]揭示赤松枯梢病不同病级针叶的内部细菌多样性及群落结构。[方法]利用高通量测序技术和生物信息学技术,对针阔混交林(赤松Pinus densiflora—麻栎Quercus acutissima)和针叶混交林(赤松—黑松Pinus thunbergii)两种林型中赤松无病斑和不同染病程度针叶内的细菌16s rRNA基因的v3-v4区进行测序和分析。[结果]针阔混交林的针叶中细菌有效序列388587条,共计679个OTU,分属28个门、69个纲、205个属;在针叶混交林的赤松针叶中的细菌有效序列336600条,共计656个OTU,分属29个门、65个纲、190个属;在针阔混交林和针叶混交林中,赤松无病斑针叶内生细菌的多样性显著高于染病针叶;无病斑针叶中相对丰度较高的内生细菌菌属为Massilia、Endobacter、Bryocella、Sphingomonas,染病针叶中乳杆菌属(Lactobacillus)、Bryocella、Endobacter、甲基杆菌属(Methylobacterium)、Singulisphaera相对丰度升高,Massilia和Sphingomonas占比下降;主成分分析结果显示,枯梢病侵染较严重的针叶细菌聚在一起,与无病斑针叶以及染病程度较轻的针叶细菌相区分。[结论]枯梢病菌的侵染影响了内生细菌的群落结构和多样性,不同混交林中赤松针叶内生细菌受枯梢病菌影响变化趋势一致,细菌多样性及相对丰度随枯梢病的加重而降低。[Objective] To reveal the endosphere bacterial diversity and community structure of needle leaves of Pinus densiflora under different disease levels. [Method] High-throughput sequencing technology was used to sequence the v3-v4 region of the bacterial 16 s rRNA gene, to determine the diversity and community structure of endophytic bacteria of P. densiflora under different disease levels. [Result] There were 388 587 effective bacterial sequences in needle leaves of coniferous-broadleaved mixed forests, totaling 679 OTUs, belonging to 28 phylum, 69 classes and 205 genera. There were 336 600 effective sequences of bacteria in needle leaves of coniferous mixed forests, totaling 656 OTUs, belonging to 29 phylum, 65 classes and 190 genera. In the coniferous-broadleaved mixed forests and coniferous mixed forests, the endophytic bacterial diversity of the asymptomatic needles of P. densiflora was higher than that of the infected needles. The predominant genera of endophytic bacteria in the asymptomatic needles were Massilia, Endobacter, Bryocella and Sphingomonas, and the relative abundance of Lactobacillus, Bryocella, Endobacter, Methylobacterium and Singulisphaera increased in infected needles, while Massilia and Sphingomonas decreased. The bacteria in coniferous needles with severe infection were distinguished from that in symptomatic coniferous needles and light infected needles. [Conclusion] The infection of S. sapinea affects the abundance of endophytic bacteria. The endophytic bacteria of the two type of mixed forests are consistent with the trend of the effects of S. sapinea, and the endophytic bacterial diversity in needles of P. densiflora is decreased gradually with the level of blight in the mixed forests.

关 键 词：赤松混交林 枯梢病 细菌多样性 群落结构 

分 类 号：S763.13[农业科学—森林保护学]