饲粮中添加不同水平锌对生长獭兔脂肪沉积及脂肪代谢相关基因表达的影响  

Effects of Dietary Different Addition Levels of Zinc on Fat Deposition and Fat Metabolism Related Genes Expression of Growing Rex Rabbits

作　　者：李淑 刘永需 张玉娟 赵红 杨国雨 刘公言 刘磊 李福昌 LI Shu;LIU Yongxu;ZHANG Yujuan;ZHAO Hong;YANG Gouyu;LIU Gongyan;LIU Lei;LI Fuchang(Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,College of Animal Science and Technology,Shandong Agricultural University,Tai’an 271018,China;Qingdao Kangda Food Co.,Ltd.,Qingdao 266555,China;Shandong Rehabilitation Hospital,Ji’nan 250109,China;Animal Husbandry and Veterinary Institute,Shandong Academy of Agricultural Sciences,Ji’nan 250100,China)

机构地区：[1]山东农业大学动物科技学院,山东省动物生物工程与疾病防治重点实验室,泰安271018 [2]青岛康大食品有限公司,青岛266555 [3]山东省康复医院,济南250109 [4]山东省农业科学院畜牧兽医研究所,济南250100

出　　处：《动物营养学报》2021年第4期2245-2252,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：现代农业产业技术体系建设专项(CARS⁃43⁃B⁃1);山东省重点研发计划(2019GNC106007);山东省博士后创新项目(2020年);新疆自治区重点研发计划项目(2020B01004⁃1⁃3)。

摘　　要：本试验旨在研究饲粮中添加不同水平锌对獭兔脂肪沉积及相关基因表达的影响。选择体重相近的3月龄獭兔160只,随机分为4组(每组40个重复,每个重复1只兔),分别饲喂添加0(对照组)、40、80和120 mg/kg锌(硫酸锌形式)的饲粮,预试期7 d,正试期49 d。结果显示:1)随着饲粮中锌添加水平的升高,生长獭兔肩胛脂肪沉积量没有显著变化(P>0.05),肾周脂肪沉积量呈现降低的趋势(P=0.0953)。2)饲粮中添加不同水平锌对肩胛脂肪和肾周脂肪中乙酰辅酶A羧化酶(ACC)、脂肪酸合成酶(FAS)、过氧化物酶体增殖物激活受体γ(PPARγ)和脂肪酸转运蛋白(FATP)以及肾周脂肪中过氧化物酶体增殖物激活受体α(PPARα)、脂肪酸结合蛋白(FABP)mRNA相对表达量均没有显著影响(P>0.05)。与对照组相比,饲粮中添加120 mg/kg锌显著提高了肩胛脂肪和肾周脂肪中肉碱脂酰转移酶1(CPT1)、激素敏感脂酶(HSL)以及肩胛脂肪中FABP mRNA相对表达量(P<0.05),显著降低了肩胛脂肪和肾周脂肪中LPL mRNA相对表达量(P<0.05)。综上所述,饲粮中添加120 mg/kg锌(硫酸锌形式)上调了生长獭兔脂质分解过程中相关基因的表达,增加了脂肪分解和脂肪酸氧化进程,从而使脂肪沉积有降低的趋势。The aim of this study was to investigate the effects of dietary with different addition levels of zinc on fat deposition and fat metabolism related genes expression of growing Rex rabbits.One hundred and sixty 3⁃month⁃old Rex rabbits with similar body weight were selected and randomly divided into 4 groups(40 repli⁃cates per group and 1 rabbit per replicate).Rabbits in the 4 groups were fed diets supplemented with 0(control group),40,80 and 120 mg/kg zinc(in the form of zinc sulfate)based on a basal diet,respectively.The pre⁃liminary feeding period sustained for 7 days,and the experimental feeding period sustained for 49 days.The re⁃sults showed as follows:1)with the increase of zinc addition level in diet,there was no significant change in the scapular fat deposition of growing Rex rabbits(P>0.05),but the perirenal fat deposition showed a decrea⁃sing trend(P=0.0953).2)Dietary with different addition levels of zinc had no significant effects on the mRNA expression levels of acetyl⁃CoA carboxylase(ACC),fatty acid synthase(FAS),peroxisome prolifera⁃tor⁃activated receptorγ(PPARγ)and fatty acid transporter(FATP)in scapular fat and perirenal fat and per⁃oxisome proliferator⁃activated receptorα(PPARα)and fatty acid binding protein(FABP)in perirenal fat(P>0.05).Compared with the control group,added with 120 mg/kg zinc in the diet significantly increased the mRNA expression levels of carnitine palmitoyl transferase 1(CPT1)and hormone sensitive lipase(HSL)in scapular fat and perirenal fat and FABP in scapular fat(P<0.05),and significantly decreased the mRNA expression levels of LPL in scapular fat and perirenal fat(P<0.05).In conclusion,dietary added with 120 mg/kg zinc(in the form of zinc sulfate)can enhance the expression of related genes in the process of lip⁃id decomposition,increase the process of lipolysis and fatty acids oxidation,so that the fat deposition has a ten⁃dence to decrease.

关 键 词：獭兔 锌 脂肪沉积 基因表达 

分 类 号：S816[农业科学—饲料科学]