高、低淀粉饲粮对山羊小肠甜味受体信号通路基因表达的影响  

Effects of High and Low Starch Diets on Gene Expressions of Key Elements of Sweet Taste Receptor Signaling Pathway in Goats

作　　者：燕爱飞 周芳芳 康劲翮[1] 王荣[1] 王敏[1] 林波[3] 冉涛[1] 刘勇[1] 谭支良[1] YAN Aifei;ZHOU Fangfang;KANG Jinhe;WANG Rong;WANG Ming;LIN Bo;RAN Tao;LIU Yong;TAN Zhiliang(Key Laboratory of Agro-Ecological Processes in Subtropical Region,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;College of Advance Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China;Department of Animal Science,Guanxi University,Nanning 530004,China)

机构地区：[1]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,湖南省畜禽健康养殖工程技术中心,长沙410125 [2]中国科学院大学现代农业科学学院,北京100049 [3]广西大学动物科学技术学院,南宁530004

出　　处：《动物营养学报》2021年第4期2278-2289,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31702139,31702141);湖南创新型省份建设专项(2020NK2012);亚热带农业生态过程重点实验室项目(2020000049)。

摘　　要：本试验旨在探讨高、低淀粉饲粮对山羊小肠黏膜结构、肠道激素浓度、甜味受体及其通路元件基因表达的影响。试验选择体况相似的雄性湘东成年黑山羊20只,随机分为2组,每组10只,分别饲喂高淀粉(50.4%,HS组)和低淀粉饲粮(13.1%,LS组)。试验期38 d。结果表明:1)与LS组相比,HS组空肠和回肠的隐窝深度增加(P>0.05),十二指肠和空肠的绒毛高度降低(P>0.05);回肠黏膜中胆囊收缩素(CCK)(P>0.05)和胰高血糖素样肽-2(GLP⁃2)(P>0.05)及回肠内容物中胰高血糖素样肽-1(GLP⁃1)(P<0.05)和酪酪肽(Pyy)(P>0.05)的浓度均增加,但回肠黏膜和回肠内容物中胃泌素抑制肽(GIP)的浓度降低(P>0.05)。2)2组间Ⅰ型味觉受体3(T1R3)、α-味移导素(α⁃gustducin)、瞬时受体电位离子通道蛋白M型5(TRPM5)、葡萄糖转运蛋白2(GLUT2)和钠/葡萄糖共转运载体1(SGLT1)在十二指肠、空肠和回肠中的基因表达量无显著差异(P>0.05),但HS组空肠α⁃gustducin基因表达量显著低于LS组(P<0.05)。3)相关性分析显示,回肠内容物葡萄糖浓度与回肠α⁃gustducin、TRPM5和SGLT1的基因表达量之间呈显著正相关(0.05<r<0.30,P<0.05),而与空肠甜味受体关键元件T1R3与SGLT1的基因表达量之间的相关系数为0.93(P<0.01),与空肠TRPM5基因表达量之间的相关系数为0.66(P<0.01),与空肠GLUT2基因表达量之间的相关系数为0.48(P<0.01)。综上所述,相比粗蛋白质和粗纤维,淀粉是甜味信号通路元件以及葡萄糖转运载体的主要影响因素,但高淀粉饲粮对发育成熟的山羊肠道黏膜结构及功能影响较小。This experiment was to explore the impact of high and low starch intake on the small intestinal mu⁃cosal structure,gastrointestinal(GI)hormone concentrations,sweet taste receptors(STR)and the key ele⁃ments of STR signaling pathway in goats.Twenty mature male Liuyang black goats were randomly allocated to 2 groups and 10 goats per group.HS group fed a high starch content(50.4%),and the LS group fed a low starch content(13.1%).The experiment lasted for 38 d.The results showed as follows:1)compared with LS group,the crypt depth of jejunum and ileum in HS group increased(P>0.05),and the villus height of duode⁃num and jejunum decreased(P>0.05);the concentrations of cholecystokinin(CCK)(P>0.05),glucagon like peptide⁃2(GLP⁃2)(P>0.05)in ileal mucosa,and glucagon like peptide⁃1(GLP⁃1)(P<0.05)and pep⁃tide YY(Pyy)(P>0.05)in ileal chyme increased,but gastrin inhibitory peptide(GIP)in ileal mucosa and chyme decreased(P>0.05).2)There was no significant difference in the gene expression of 3 typeⅠtaste receptors(T1R3),α⁃gustducin,transient receptor potential ion channel protein M type 5(TRPM5),glucose transporter 2(GLUT2)and sodium/glucose co⁃transporter 1(SGLT1)in duodenum,jejunum and ileum(P>0.05).In jejunum,gene expression ofα⁃gustducin in LS group was significantly higher than that in HS group(P<0.05).3)Correlation analysis showed that ileal glucose concentration was positively correlated with gene expressions ofα⁃gustducin,TRPM5 and SGLT1 of ileal(0.05<r<0.30,P<0.05).In addition,the gene ex⁃pression of T1R3 in jejunum was high related(P<0.01)to that of SGLT1,TRPM5 and GLUT2 in jejunum with the correlation efficiency as 0.93(P<0.01),0.66(P<0.01)and 0.48(P<0.01),respectively.In con⁃clusion,compared with the crude protein and fiber in diets,starch is the major factor on the gene expressions of sweet taste receptors and glucose transporters,but with a little effect on the intestinal mucosal structure.

关 键 词：淀粉饲粮 甜味受体 肠道激素 基因表达 

分 类 号：S826[农业科学—畜牧学]