肉牛血清基质金属蛋白酶2、9、13浓度与血清抗氧化指标及肉品质指标的相关关系  

Correlation between Serum Matrix Metalloproteinases 2,9 and 13 Concentrations and Serum Antioxidant Indexes,Meat Quality of Beef Cattle

作　　者：冯鑫 栾嘉明 李月明[1] 周金影 金英海[1] 张敏[1] 耿春银[1] FENG Xin;LUAN Jiaming;LI Yueming;ZHOU Jinying;JIN Yinghai;ZHANG Min;GENG Chunyin(College of Agriculture,Yanbian University,Yanji 133000,China)

机构地区：[1]延边大学农学院,延吉133000

出　　处：《动物营养学报》2021年第4期2347-2354,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金项目(31660669);吉林省“十三五”科研规划项目(JJKH20180904KJ)。

摘　　要：本研究旨在揭示肉牛血清基质金属蛋白酶(MMP)2、9、13浓度与血清抗氧化指标和肉品质指标的相关关系。试验选取健康状况良好、体重相近的杂交育肥公牛[西门塔尔牛×延边黄牛,初始体重(436±35)kg]52头,饲喂相同的全混合日粮(TMR)。饲养试验结束前1天在晨饲前采集每头牛的血液,制备血清,测定MMP2、9、13浓度以及抗氧化指标;饲养145 d后全部屠宰,测定屠宰性能、胴体性状及肉品质指标。结果显示:肉牛血清MMP2浓度与血清活性氧自由基(ROS)、丙二醛(MDA)含量,谷胱甘肽过氧化物酶(GSH⁃Px)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)活性及总抗氧化能力(T⁃AOC)均无显著相关关系(P>0.05);肉牛血清MMP9浓度与血清ROS含量显著负相关(r=-0.358,P=0.011),与血清其他抗氧化指标无显著相关关系(P>0.05);肉牛血清MMP13浓度与血清ROS含量显著负相关(r=-0.292,P=0.040),与血清其他抗氧化指标也无显著相关关系(P>0.05)。肉牛血清MMP2浓度与牛肉大理石花纹评分(r=0.352,P=0.012)和肌内脂肪含量(r=0.307,P=0.036)显著正相关;肉牛血清MMP9浓度与牛肉剪切力显著负相关(r=-0.430,P=0.002);肉牛血清MMP13浓度与牛肉剪切力显著负相关(r=-0.281,P=0.048);肉牛血清MMP2、9、13浓度与牛肉饱和脂肪酸(SFA)、单不饱和脂肪酸(MUFA)、多不饱和脂肪酸(PUFA)、n⁃3 PUFA、n⁃6 PUFA比例以及n⁃6/n⁃3、PUFA/SFA、C18∶2n6/C18∶3n3均无显著相关关系(P>0.05)。由此可见,肉牛血清MMP2浓度与牛肉大理石花纹评分显著正相关,血清MMP9、13浓度与血清ROS含量及牛肉剪切力显著负相关,血清MMP2、9、13浓度可成为潜在的生物标记物用于肉牛肉质性状的遗传选择或肉品质调控策略。The purpose of this study was to reveal the correlation between serum concentration of matrix metal⁃loproteinases(MMP)2,9 and 13 and serum antioxidant indexes capacity,meat quality of beef cattle.In the experiment,fifty⁃two healthy crossbred bulls(Simmental×Yanbian cattle)with similar body weight[initial body weight was(436±35)kg]were selected,and the cattle were fed the same total mixed ration(TMR).On 1 day before the end of feeding experiment,blood of each cattle was sampled in the morning before feeding to prepare serum for measuring MMP2,9 and 13 concentrations and antioxidant indexes.After 145 days of feeding,all cattle were slaughtered,and the indexes of slaughter performance,carcass traits and meat quality were measured.The results showed that the serum MMP2 concentration of cattle had no significant correlation with serum reactive oxide species(ROS)and malondialdehyde(MDA)contents,glutathione peroxidase(GSH⁃Px),catalase(CAT),superoxide dismutase(SOD),glutathione reductase(GR)activities and total antioxidant capacity(T⁃AOC)(P>0.05).Serum MMP9 concentration of cattle was significantly negatively correlated with serum ROS content(r=-0.358,P=0.011),but was not significantly correlated with other se⁃rum antioxidant indexes(P>0.05).Serum MMP13 concentration of cattle was significantly negatively correla⁃ted with serum ROS content(r=-0.292,P=0.040),but had no significant correlation with other serum an⁃tioxidant indexes(P>0.05).Serum MMP2 concentration of cattle was significantly positively correlated with beef marble score(r=0.352,P=0.012)and intramuscular fat content(r=0.307,P=0.036).Serum MMP9 concentration of cattle was significantly negatively correlated with beef shear force(r=-0.430,P=0.002).Serum MMP13 concentration of cattle was negatively correlated with beef shear force(r=-0.281,P=0.048).There was no significant correlation between MMP2,9 and 13 concentrations and the proportions of saturated fatty acids(SFA),monounsaturated fatty acids(MUFA),polyunsaturated fatty acids(PUFA),n⁃3 PUF

关 键 词：肉牛 基质金属蛋白酶 抗氧化指标 肉品质 相关关系 

分 类 号：S823.9[农业科学—畜牧学]