不同他克莫司血药浓度检测方法的对比分析  

Comparative analysis of different methods of tacrolimus blood concentration detection

作　　者：郭奉洁[1] 马锡慧 李彬钰 孙玉洁 Guo Fengjie;Ma Xihui;Li Binyu;Sun Yujie(Basic Research Laboratory,the eighth medical center of PLA general hospital,Beijing 100091,China;Beijing Key Laboratory of Immunology Regulatory and Organ Transplantation,Beijing 100091,China.)

机构地区：[1]解放军总医院第八医学中心卫勤部,北京100091 [2]解放军总医院第八医学中心原移植研究室,北京100091

出　　处：《实用器官移植电子杂志》2021年第2期116-119,共4页Practical Journal of Organ Transplantation(Electronic Version)

基　　金：军队医学科技青年培育计划(18QNP035);国家自然科学基金(81571555)。

摘　　要：目的分析化学发光微粒子免疫法和胶乳增强免疫抑制法检测他克莫司(Tacrolimus,Tac)全血药物浓度的结果。方法用化学发光微粒子免疫法和胶乳增强免疫抑制法平行检测96例肾移植受者他克莫司全血药物浓度,对比检测结果差异性并进行相关和回归分析。结果采用化学发光微粒子免疫法测得的Tac血药浓度(5.78±2.18)μg/L低于采用胶乳增强免疫抑制法测得的Tac血药浓度(6.51±2.32)μg/L,且差异具有统计学意义(P<0.001);两种方法检测Tac血药浓度结果呈显著正相关(r=0.9676,P<0.0001);两种方法检测Tac血药浓度结果符合一元线性回归方程Y=0.551+1.032X(其中X为化学放光微粒子免疫法,Y为胶乳增强免疫抑制法)。结论两种方法检测Tac血药浓度具有很好的相关性,但差异显著,不可相互比较,二者可通过一元线性回归方程来互相推算,建议同一患者长期选用同一种方法监测Tac血药浓度,以便为临床提供准确可靠的个体化用药依据。Objective To analyze the results of the determination of Tacrolimus(Tac)concentration in whole blood by chemiluminescent microparticle immunoassay and latex enhanced immunosuppression.Methods The whole blood concentration of tacrolimus in 96 renal transplant recipients was parallelly measured by chemiluminescent microparticle immunoassay and latex enhanced immunosuppression,the results of different tests were compared and the correlation and regression were analyzed.Results The concentration of tacrolimus measured by chemiluminescent microparticle immunoassay(5.78±2.18)μg/L was lower than that measured by latex enhanced immunosuppression(6.51±2.32)μg/L,and the difference was statistically significant(P<0.001).There was a significant positive correlation between the two methods(r=0.9676,P<0.0001),besides,the results of the two methods were in accordance with the linear regression equation Y=0.551+1.032X(X was the chemical radioparticle immunoassay,Y was the latex enhanced immunosuppression).Conclusion There was a good correlation between the two methods,but due to the significant difference,comparison between each other cannot be made.The two methods can be calculated by linear regression equation.It is suggested that the same method should be selected for the same patient to monitor the whole blood concentration of tacrolimus in the long run,so as to provide accurate and reliable basis for clinical individual drug application.

关 键 词：化学发光微粒子免疫法 胶乳增强免疫抑制法 他克莫司 肾移植 

分 类 号：R969.1[医药卫生—药理学]