Effect of oligofructose on resistance to postoperative high-fat diet-induced damage of metabolism in diabetic rats after sleeve gastrectomy  

作　　者：Ming-Wei Zhong Yue Li Yu-Gang Cheng Qiao-Ran Liu San-Yuan Hu Guang-Yong Zhang 

机构地区：[1]Department of General Surgery,The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital,Jinan 250014,Shandong Province,China [2]Key Laboratory of Metabolism and Gastrointestinal Tumor,The First Affiliated Hospital of Shandong First Medical University,Jinan 250014,Shandong Province,China [3]Key Laboratory of Laparoscopic Technology,The First Affiliated Hospital of Shandong First Medical University,Shandong Medicine and Health Key Laboratory of General Surgery,Jinan 250014,Shandong Province,China [4]Department of General Surgery,Shandong Qianfoshan Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250014,Shandong Province,China

出　　处：《World Journal of Diabetes》2021年第4期453-465,共13页世界糖尿病杂志（英文版）（电子版）

基　　金：Supported by The National Natural Science Foundation of China,No. 81900705。

摘　　要：BACKGROUND Sleeve gastrectomy(SG) can induce prominent remission of type 2 diabetes mellitus.However,the long-term remission rate of diabetes usually decreases over time.Oligofructose has been verified to modulate host metabolism.The aim of this study was to explore the protective effect of oligofructose on high-fat diet(HFD)-induced metabolic dysfunction after SG.AIM To study the effect and mechanism of oligofructose on diabetic remission in diabetic rats after SG.METHODS SG and SHAM operation were performed on diabetes rats induced with an HFD,nicotinamide,and low-dose streptozotocin.Then the rats in the SHAM and SG groups were continuously provided with the HFD,and the rats in sleeve gastrectomy-oligofructose group were provided with a specific HFD containing10% oligofructose.Body weight,calorie intake,oral glucose tolerance test,homeostasis model assessment of insulin resistance,lipid profile,serum insulin,glucagon-like peptide 1(GLP-1),total bile acids,lipopolysaccharide(LPS),and colonic microbiota levels were determined and compared at the designated time points.All statistical analyses were performed using Statistic Package for Social Science version 19.0(IBM,United States),and the statistically significant difference was considered at P <0.05.RESULTS At 2 wk after surgery,rats that underwent SG exhibited improved indexes of glucose and lipid metabolism.Compared with the SG group,the rats from SGoligofructose group exhibited better parameters of glucose and lipid metabolism,lower body weight(526.86±21.51 vs 469.25±21.84,P <0.001),calorie intake(152.14±9.48 vs 129.63±8.99,P <0.001),homeostasis model assessment of insulin resistance(4.32±0.57 vs 3.46±0.52,P <0.05),and LPS levels(0.19±0.01 vs 0.16±0.01,P <0.05),and higher levels of insulin(1.17±0.17 vs 1.58±0.16,P <0.001) and GLP-1(12.39±1.67 vs 14.94±1.86,P <0.001),and relative abundances of Bifidobacterium(0.0034±0.0014 vs 0.0343±0.0064,P <0.001),Lactobacillus(0.0161±0.0037 vs 0.0357±0.0047,P <0.001),and Akkermansia muciniphila(0.0050±0.002

关 键 词：Sleeve gastrectomy OLIGOFRUCTOSE DIABETES Gut microbiota LIPOPOLYSACCHARIDE Glucagon-like peptide 1 

分 类 号：R656.61[医药卫生—外科学]