山羊鼻内肿瘤病毒荧光定量PCR检测方法的建立及应用  

Development and Application of a Fluorescent PCR Method for Detection of Enzootic Nasal Tumor Virus

作　　者：肖霜艳 翟少伦 陈小文 谢逸伦 周秀蓉 吕殿红 温肖会 翟颀 贾春玲 魏文康 刘正飞[1] XIAO Shuangyan;ZHAI Shaolun;CHEN Xiaowen;XIE Yilun;ZHOU Xiurong;LYV Dianhong;WEN Xiaohui;ZHAI Qi;JIA Chunling;WEI Wenkang;LIU Zhengfei(College of Animal Medicine,Huazhong Agricultural University,Wuhan 430000,China;Jnstitute of Animal Health,Guangdong Academy of Agricultural Sciences,Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Ministry of Agriculture and Rural Affairs,Key Laboratory of Anim al Disease Prevention of Guangdong province,Guangzhou 510640,China;Sihui Animal Husbandry and Veterinary Bureau,Sihui 526200,China)

机构地区：[1]华中农业大学动物医学院,武汉430000 [2]广东省农业科学院动物卫生研究所,广东省畜禽疫病防治研究重点实验室,农业农村部兽用药物与诊断技术广东科学观测实验站,广州510640 [3]四会市畜牧兽医局,四会526200

出　　处：《中国动物传染病学报》2021年第2期9-14,共6页Chinese Journal of Animal Infectious Diseases

基　　金：广东省重点研发项目(2019B020217002);广东省现代农业产业技术体系创新团队(2019KJ114);广东省农业科学院学科团队项目(201634TD)。

摘　　要：为了解山羊鼻内肿瘤病毒(ENTV-2)的流行情况,本研究针对ENTV-2的env基因设计了1对特异性引物与探针,通过RT-PCR条件优化,成功建立了ENTV-2荧光RT-qPCR检测方法。该方法特异性高、灵敏性强(5.61 copies/μL)、重复性好(CV为0.09%~1.20%)。本研究建立的一步法荧光RT-qPCR方法为ENTV-2的临床检测以及流行病学调查提供技术支撑。In order to understand the prevalence of Enzootic nasal tumor virus of goats(ENTV-2)and to distinguish between Exogenous retroviruses and Endogenous retroviruses in goats,a fluorescent PCR method was developed in this study.The results showed that this method had high specificity,high sensitivity(5.61 copies/μL)and good reproducibility(the range of CV was 0.09%-1.20%).The RT-qPCR method developed here provided technological support for ENTV-2 detection and epidemiological investigation.

关 键 词：山羊鼻内肿瘤病毒(ENTV-2) ENV基因 荧光PCR 诊断 

分 类 号：S852.659.3[农业科学—基础兽医学]