侵蚀黑土玉米和大豆根部伴生细菌群落结构分析  

Communities Structure of Corn and Soybean Root-associated Bacteria in Eroded Black Soil

作　　者：喻江[1,2] 范国权[3] IKENAGA Makoto SAKAI Masao 王光华[1] 刘晓冰[1] 于镇华[1] YU Jiang;FAN Guo-quan;IKENAGA Makoto;SAKAI Masao;WANG Guang-hua;LIU Xiao-bing;YU Zhen-hua(Northeast Institute of Geography and Agroecology/Key laboratory of Mollisoals Agroecology.Chinese Academy of Sciences,Harbin 150081,China;Harbin Universily of Commerce,Harbin 150028,China;Research Institute of Potatoes,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Faculy of Agriculture,Kagoshima University,Kagoshima 890-0065,Japan)

机构地区：[1]中国科学院东北地理与农业生态研究所/黑土区农业生态重点实验室,黑龙江哈尔滨150081 [2]哈尔滨商业大学,黑龙江哈尔滨150028 [3]黑龙江省农业科学院马铃薯研究所,黑龙江哈尔滨150086 [4]日本鹿儿岛大学农学院,日本鹿儿岛890-0065).

出　　处：《大豆科学》2021年第2期232-240,共9页Soybean Science

基　　金：黑龙江省自然科学基金(D2018008);中国科学院青年创新促进会项目(2019233);哈尔滨商业大学博士科研启动项目(2019DS103)。

摘　　要：为分析东北侵蚀黑土中大豆和玉米根部伴生细菌群落结构多样性,促进土壤侵蚀过程中其根部伴生细菌群落结构响应规律的研究,本研究结合LNA-PCR技术和高通量测序方法,模拟东北黑土侵蚀土壤,分析玉米播种期和抽穗期、大豆开花期根部细菌群落结构多样性和差异。结果表明:土壤表层剥离后大豆根部细菌群落多样性降低,而玉米根部细菌群落多样性变化不显著。变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)为大豆和玉米根部伴生细菌共有的优势菌门。属水平上,Acinetobacter、Rhodanobacter、Steroidobacter和Dyella主要在玉米根部获得,而Bacillus、Arenimonas、Variovorax、Propionibacterium、gnorankp和CandidatedivisionTM7主要在大豆根部发现。本研究首次发现Candidate division TM7也是植物伴生细菌的成员,其序列主要划分为α、β和γ 3个簇,有97.5%的序列属于α簇。研究证实了植物根部伴生细菌群落结构不仅具有宿主特异性,且易受土壤侵蚀影响。In order to analyze the response of root-associated bacterial diversity to soil erosion, the diversity and differences of bacterial community structure in the simulated eroded black soil in northeast China during the sowing and heading periods of maize and the flowering periods of soybean were investigated. Locked nucleic acid(LNA) oligonucleotide-PCR clamping and high-throughput sequencing were used. The results showed that the diversity indices of soybean root-associated bacterial communities declined with soil erosion, while those of corn root-associated bacterial communities did not change. The dominant root-associated bacterial phyla were Proteobacteria, Actinobacteria, Bacteroidetes and Firmicutes both in soybean and corn samples. On the genus level, Acinetobacter, Rhodanobacter, Steroidobacter and Dyella were mainly found in corn roots, whereas Bacillus, Arenimonas, Variovorax, Propionibacterium and gnorankpCandidatedivisionTM7 were mainly detected in soybean roots. Among them, Candidate Division TM7 was found to be a member of root-associated bacteria for the first time in this study and all sequences were grouped into three major clusters(α, β and γ). Approximately 97.5% of Candidate Division TM7 sequences obtained in this study divided into cluster α. Taken together, root-associated bacterial communities were host specific and sensitive to soil erosion.

关 键 词：LNA-PCR 高通量测序 土壤侵蚀 根部伴生细菌 群落结构 

分 类 号：S513[农业科学—作物学] S565.1