4个紫花苜蓿品种对低温胁迫的生理响应及抗寒性评价  

Evaluation of physiological response and cold resistance of four alfalfa cultivars to low temperature stress

作　　者：赵一航 孟令东 张晓萌 王丽娜[1] 刘皓玥 毕琳琳 刘宏亮 殷秀杰[1] ZHAO Yihang;MENG Lingdong;ZHANG Xiaomeng;WANG Li'na;LIU Haoyue;BI Linlin;LIU Hongliang;YIN Xiujie(Department of Grass Science College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;Nestle Dairy Farming Institute,Harbin 150110,Heilongjiang,China;Nestle Grain Competence Center,Harbin 150110,Heilongjiang,China)

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030 [2]雀巢奶牛养殖培训中心,黑龙江哈尔滨150110 [3]雀巢谷物能力中心,黑龙江哈尔滨150110

出　　处：《草业科学》2021年第4期683-692,共10页Pratacultural Science

基　　金：规模化人工草地种植结合循环模式研究与示范(2017YFD0502106);雀巢奶牛养殖培训中心合作项目;国家自然科学基金(31802120);东北农业大学“学术骨干”基金项目。

摘　　要：本研究选取‘肇东’、‘龙牧801’、‘东农1号’、‘敖汉’4种紫花苜蓿(Medicago sativa)作为研究对象,进行低温胁迫试验,通过对生理生化指标和叶片解剖结构的观测分析,综合比较了4种紫花苜蓿的抗寒性。结果表明:低温胁迫下,4种紫花苜蓿相对电导率、丙二醛含量、可溶性糖含量、游离脯氨酸含量、过氧化物酶和超氧化物歧化酶活性与对照相比有所上升,其中‘肇东’各个指标的上升幅度均最大。通过隶属函数综合分析得出,4种紫花苜蓿的抗寒性为‘肇东’>‘龙牧801’>‘东农1号’>‘敖汉’。通过观测4种苜蓿的解剖结构发现,抗寒性较强的‘肇东’和‘龙牧801’的叶片厚度、栅栏组织厚度和海绵组织厚度小于抗寒性较弱的‘敖汉’,且细胞结构更为紧密。Four alfalfa(Medicago sativa)cultivars,‘Zhaodong’,‘Longmu 801’,‘Dongnong No.1’,and‘Aohan’,were studied.The cold resistance of the four alfalfa cultivars was comprehensively compared by analyzing physiological biochemical indexes and leaf anatomical structure.The relative electrical conductivity,malondialdehyde content,soluble sugar content,free proline content,peroxidase,and superoxide dismutase activity of the alfalfa cultivars increased compared with the control.Among them,‘Zhaodong’had the highest increase in each indicator.Through a comprehensive analysis of the membership function,it was concluded that the cold resistance of the alfalfa cultivars from high to low is as follows:‘Zhaodong’>‘Longmu 801’>‘Dongnong No.1’>‘Aohan’.By observing the anatomical structure of the alfalfa cultivars,it was found that the blade thickness,palisade tissue thickness,and sponge tissue thickness of‘Zhaodong’and‘Longmu 801’with strong cold resistance were less than that of‘Aohan’,which was less cold resistant,and the cell structure was tighter than that of‘Aohan’.

关 键 词：紫花苜蓿 抗寒性 解剖结构 品种比较 

分 类 号：S541.9[农业科学—作物学]