近红外荧光探针检测金属离子、小分子和生物大分子  

Detection of Metal Ions,Small Molecules and Large Molecules by Near-Infrared Fluorescent Probes

作　　者：刘园园 郭芸 罗晓刚 刘根炎 孙琦 Yuanyuan Liu;Yun Guo;Xiaogang Luo;Genyan Liu;Qi Sun(Key Laboratory for Green Chemical Process of Ministry of Education,School of Chemical Engineering andPharmacy,Wuhan Institute of Technology,Wuhan 430205,China;School of Chemistry and EnvironmentalEngineering,Wuhan Institute of Technology,Wuhan 430205,China;School of Materials Science andEngineering,Zhengzhou University,Zhengzhou 450001,China)

机构地区：[1]武汉工程大学化工与制药学院绿色化工过程教育部重点实验室,武汉430205 [2]武汉工程大学化学与环境工程学院,武汉430205 [3]郑州大学材料科学与工程学院,郑州450001

出　　处：《化学进展》2021年第2期199-215,共17页Progress in Chemistry

基　　金：国家自然科学基金项目(No.21807082);中央引导地方科技发展资金(No.2020ZYYD040);湖北省科技厅科学技术研究项目(No.2020BAB073);湖北省教育厅科学技术研究项目(No.T201908,Q20171503);湖北省教育厅教学研究项目(No.2017327)资助。

摘　　要：荧光探针技术是近年来发展迅速的一种荧光分析方法,具有灵敏度高、选择性好、操作简便和响应迅速等特点,受到环境及生命科学领域的青睐。随着荧光探针技术的发展,近红外一区荧光探针由于具有发射波长长(600~900 nm)、对细胞损伤小、组织穿透性强和自发荧光背景低等优点,被广泛应用于细胞、组织等复杂生物体系中生物分子的检测、示踪及成像。本文评述了近年来(2016~2020年)近红外荧光探针对金属离子(Hg^(2+)、Cu^(2+)、Zn^(2+)、Al^(3+)、Fe^(3+))、生物小分子(Cys、N_(2)H_(4)、H_(2)S、H_(2)O_(2))与生物大分子(亮氨酸氨基肽酶、β-半乳糖苷酶)等重要生物分子的检测及成像的研究进展,讨论了该类探针在细胞及活体的分析应用,并对近红外荧光探针的前景进行了展望。Fluorescent probe analysis,as a rapidly developed analytical method with high sensitivity,good selectivity,and fast responsibility,has been favored in environmental and life science field.And near-infrared fluorescent probe is one of the most developed fluorescent probes.It has been widely employed in detection,tracking and imaging of biomolecules in complex biological systems such as cells and tissues due to its distinguished features including long emission wavelength(600 to 900 nm),low cell damage,strong tissue penetration and low spontaneous fluorescence background.In this review,we summarize the development of near-infrared fluorescent probes in detection and imaging of metal ions(Hg^(2+),Cu^(2+),Zn^(2+),Al^(3+),Fe^(3+)),small biological molecules(Cys,N_(2)H_(4),H_(2)S,H_(2)O_(2)),biological macromolecules(Leucine aminopeptidase,β-galactosidase)and other important biological molecules in recent five years.We further give the in-depth discussion on the in vitro and in vivo analytical applications of near-infrared fluorescent probes,and propose their future perspectives.

关 键 词：近红外荧光探针 金属离子 大分子 小分子 生物成像 

分 类 号：O657[理学—分析化学]