肥胖2型糖尿病模型OLETF大鼠不同病程阶段肝脏水通道蛋白9 mRNA的表达  

Expression of hepatic aquaporin 9 mRNA in OLETF rats with obesity type 2 diabetes mellitus at different stages

作　　者：段玉敏[1] 袁振芳[2] DUAN Yumin;YUAN Zhenfang(Department of Endocrinology,The Sisth People's Hospital of Beijing,Beijing 100007,China;Department of Endocrinology,The First Hospital of Peking University,Beijing 100034,China)

机构地区：[1]北京市第六医院内分泌科,北京100007 [2]北京大学第一医院内分泌科,北京100034

出　　处：《安徽医药》2021年第5期1003-1006,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的观察肥胖2型糖尿病模型OLETF大鼠在不同糖尿病病程阶段肝脏组织水通道蛋白9(AQP 9)mRNA表达的变化,探讨AQP 9在肥胖及糖尿病发病中的作用。方法取OLETF大鼠30只为实验组,同种系的非糖尿病LETO大鼠18只为正常对照组。8周(基线)时两组大鼠分别处死6只。然后将OLETF大鼠分为未治疗组(OLETF组)12只及盐酸二甲双胍治疗组(OLETF/M组)12只。分别观察每组大鼠在8周、18周、28周时的体质量、血清三酰甘油、胆固醇,行葡萄糖耐量试验测定血糖与胰岛素水平,用Real-time PCR测定肝脏组织AQP9 mRNA的表达。结果OLETF组在18周均患糖尿病,28周的血糖水平与18周大致相当,随周龄增加其体质量、血糖、胰岛素、血清三酰甘油、胆固醇均明显增高。肝脏组织AQP 9 mRNA表达在8周、18周及28周龄分别是同周龄LETO组的0.9倍,1.0倍及0.7倍。OLETF/M组在18周均患糖尿病,但血糖水平低于OLETF组;28周血糖均恢复为糖耐量异常。OLETF/M组的体质量、胰岛素水平与OLETF组差异无统计学意义,三酰甘油、胆固醇水平较OLETF组低。肝脏组织AQP 9 mRNA表达在8周、18周及28周龄分别是同周龄LETO组的0.9倍,0.6倍及0.5倍。结论OLETF大鼠随周龄增加,肥胖、高脂血症、胰岛素抵抗加重,血糖增高。OLETF组和OLETF/M组的肝脏组织的AQP 9 mRNA表达均呈先上调后下调的趋势,提示脂肪分解先增强后减弱。OLETF/M组肝脏组织AQP 9的mRNA表达水平均较同周龄OLETF组低。盐酸二甲双胍可以改善OLETF/M组大鼠的糖脂代谢紊乱并抑制肝脏AQP 9 mRNA的表达。肝脏AQP 9在肥胖和糖尿病的病程中起一定的作用。Objective To observe the changes of aquaporin 9(AQP9)mRNA expression in liver in the spontaneous type 2 diabetes animal model OLETF(Otsuka Long-Evans Tokushima Fatty)rats at different stages and to explore the role of AQP9 in obesity development and diabetes.Methods OLETF rats(n=30)were studied,with same strains of non-diabetic Long-Evans Tokushima Otsuka(LETO)rats(n=18)used as age-matched normal controls.At 8 weeks(baseline),6 rats in each of the two groups were sacrificed.Then OLETF rats were divided into no treatment group(OLETF group,n=12)and metformin hydrochloride treatment group(OLETF/M group,n=12).At the age of 8 weeks,18 weeks and 28 weeks,body weights were obtained,biochemical items were measured,including serum triglyceride,cholesterol,glucose,insulin level with oral glucose tolerance test(OGTT).Liver were obtained for the measurement of AQP9 mRNA expression by real-time PCR.Results 1.Rats in OLETF group developed diabetes at 18 weeks,the glucose were almost same level at 28 weeks with 18 weeks.Body weight and biochemical items including glucose and insulin of OGTT,serum triglyceride,serum cholesterol,were increased with rats’age.At the age of 8 weeks,18 weeks and 28 weeks,liver AQP9 mRNA expression in OLETF group were decreased 0.9 times,1.0 times and 0.7 times.2.Rats OLETF/M group developed diabetes at 18 weeks,but the glucose level was lower than that of age-matched OLETF group rats.At 28 weeks,the rats’glucose improved,rats returned to impaired glucose tolerance.The body weight and insulin of OGTT of OLETF/M group rats were of no significant difference with those of the OLETF group rats.Biochemical items including serum triglyceride,serum cholesterol were lower than those of the OLETF groups rats.At the age of 8 weeks,18 weeks and 28 weeks,liver AQP9 mRNA expression in the OLETF/M group were decreased 0.9 times,0.6 times and0.5 times.Conclusion The degrees of obesity,dyslipidemia,insulin resistance and glucose of OLETF rats are significantly increased with rats’age.LiverAQP9 mRNA expression a

关 键 词：水通道蛋白9 糖尿病 2型 肥胖 大鼠 近交OLETF 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]