乌贼黑色素的提取及与金属离子络合性能的综合性实验设计  

Design on comprehensive experiment for extraction of sepia melanin and its complexation with metal ions

作　　者：陈翠霞 赵玉荣 李慧 李阳 CHEN Cuixia;ZHAO Yurong;LI Hui;LI Yang(Chemical Engineering College,China University of Petroleum(East China),Qingdao 266580,China)

机构地区：[1]中国石油大学(华东)化学工程学院,山东青岛266580

出　　处：《实验技术与管理》2021年第3期79-82,共4页Experimental Technology and Management

基　　金：中央高校自主创新项目(18CX05012A);中国石油大学(华东)教学改革项目(YKC2019007,YJ-K202007,YJ-Q202002)。

摘　　要：该文设计了乌贼黑色素的提取及其性质表征的综合性实验。利用水洗法、酸水解法和碱水解法从新鲜的乌贼墨囊中提取黑色素,采用透射电子显微镜、红外光谱仪和紫外-可见漫反射光谱仪等精密仪器表征提取黑色素的结构特征,分析乌贼黑色素与Fe^(3+)、Pb^(2+)、Cu^(2+)等金属离子的络合作用。该综合性实验有助于学生了解生物大分子的提纯方法,掌握分析生物大分子结构和功能的技术手段和方法,明确黑色素与金属离子络合的原理,既可以激发学生的科研热情,又可以提升学生的综合实践能力和创新思维能力。A comprehensive experiment on the extraction and characterization of sepia melanin is designed. Melanin is extracted from fresh cuttlefish black bag by water washing, acid hydrolysis and alkaline hydrolysis. Transmission electron microscopy, infrared spectroscopy and ultraviolet visible diffuse reflectance spectroscopy are used to characterize the structure of the extracted melanin. The metal chelating properties of sepia melanin with metal ions such as Fe^(3+), Pb^(2+) and Cu^(2+) are analyzed. This comprehensive experiment helps students to understand the purification method of biological macromolecules, master the technical methods and to analyze the structure and function of biological macromolecules, and clarify the principles of melanin and metal ion complexation, which can not only stimulate students′ enthusiasm for scientific research, but also improve their comprehensive practical ability and innovative thinking ability.

关 键 词：综合性实验设计 黑色素 金属离子络合 

分 类 号：O633[理学—高分子化学]