蟾酥注射液对人肝癌SMMC-7721细胞增殖与凋亡的影响  

Effects of Venenum Bufonis Injection on the Proliferation and Apoptosis of Human Liver Cancer SMMC-7721 Cells

作　　者：赖慧 赖洪林 陈立 任维 杨思进 赵福兰 Lai Hui;Lai Honglin;Chen Li(Affiliated Traditional Chinese Medicine Hospital,Southwest Medical University,Sichuan 646000,China)

机构地区：[1]西南医科大学附属中医医院药剂科,泸州646000

出　　处：《医学研究杂志》2021年第4期69-73,共5页Journal of Medical Research

基　　金：四川省教育厅科研项目(18ZB0643)。

摘　　要：目的研究蟾酥注射液对人肝癌SMMC-7721细胞增殖与凋亡的影响。方法CCK-8法检测SMMC-7721细胞活力;DCFH-DA染色法检测SMMC-7721细胞活性氧(reactive oxygen species,ROS)水平;荧光染色法观察SMMC-7721细胞线粒体膜电位变化;流式细胞术检测SMMC-7721细胞凋亡率。结果蟾酥注射液明显抑制SMMC-7721细胞增殖,呈时间和浓度依赖性,作用24、48和72h后,半数抑制浓度(IC 50)分别为2.60±0.01、2.00±0.10和1.57±0.17μg/L;ROS检测结果显示不同浓度药物处理组细胞内ROS水平与对照组比较均明显升高(P=0.000);荧光染色观察到药物处理组细胞的绿色荧光随药物浓度增加而变强,红色荧光反而逐渐变弱;流式细胞术检测出SMMC-7721细胞经低、中、高浓度蟾酥注射液处理48h后,总凋亡率分别为17.33%±3.20%、25.60%±1.05%和35.76%±4.72%,与对照组比较,总凋亡率明显增加,呈浓度依赖性,差异有统计学意义(P<0.05)。结论蟾酥注射液能显著抑制人肝癌SMMC-7721细胞增殖,并诱导7721细胞凋亡。该凋亡可能与蟾酥注射液引起SMMC-7721细胞ROS水平增加、线粒体损伤以及膜电位下降有关。Objective To observe the effects ofvenenumbufonis injection on the proliferation and apoptosis ofhuman liver cancer SMMC-7721 cells.Methods CCK-8 assay was performed to detect the cell viability of SMMC-7721 cells.DCFH-DA staining method was adopted to investigate the changes of the reactive oxygen species(ROS)level in SMMC-7721 cells;Fluorescence staining method was used to observe the changes of mitochondrial membrane potential in SMMC-7721 cells.Flow cytometry was used to detect the apoptosis rate of SMMC-7721 cells.Results After being treated with different concentrations of venenumbufonis injection for 24,48 and 72h,the half inhibition concentrations(IC 50)of SMMC-7721 cells were 2.60±0.01,2.00±0.10 and 1.57±0.17μg/L,respectively.Venenumbufonis injection inhibited the proliferation of SMMC-7721 cells significantly in a time-and concentration-dependent manner.ROS detection results showed that the intracellular ROS levels in different concentrations of drug treatment groups were significantly increased compared with the control group(P=0.000).According to fluorescence staining results,the green fluorescence intensity of the treated cells increased with the increase of drug concentration,while the red fluorescence intensity decreased.After treated with low,medium and high concentrations of venenumbufonis injection for 48h,the totalapoptotic rates of SMMC-7721 cells were 17.33%±3.20%,25.60%±1.05%and 35.76%±4.72%,respectively.Compared with the control group,the apoptotic rates increased significantlyin a dose dependent manner,and the difference was statistically significant(P<0.05).Conclusion Venenumbufonis injection can inhibit the proliferationof human liver cancer SMMC-7721 cells and induce SMMC-7721 cell apoptosis.The mechanism of which may be related to the increase of ROS level,mitochondrialdamage and decrease of membrane potential in SMMC-7721 cells caused by venenumbufonis injection.

关 键 词：蟾酥注射液 肝癌 SMMC-7721 增殖 凋亡 

分 类 号：R979.1[医药卫生—药品]